p-Nitrophenyl 3,4,6-Tri-O-acetyl-β-D-galactopyranoside (CAS 62346-04-7)

p-Nitrophenyl 3,4,6-Tri-O-acetyl-β-D-galactopyranoside is extensively used in biochemical research, particularly in the study of glycosidase enzymes and carbohydrate chemistry. Its acetylated β-D-galactopyranoside structure mimics glycan substrates, making it a valuable tool for investigating enzyme kinetics and substrate specificity of galactosidases. This compound serves as a substrate analog, aiding in the elucidation of the mechanisms underlying glycoside hydrolysis and facilitating the assessment of glycosidase inhibitors. Researchers utilize p-Nitrophenyl 3,4,6-Tri-O-acetyl-β-D-galactopyranoside as a standard reagent in various biochemical assays aimed at quantifying and characterizing glycosidase activity. Its nitrophenyl moiety allows for colorimetric detection of enzymatic hydrolysis reactions, enabling convenient monitoring of enzyme activity levels. Furthermore, this compound finds applications in the synthesis of complex glycoconjugates and glycans, contributing to the development of carbohydrate-based probes and glycobiology research. Overall, p-Nitrophenyl 3,4,6-Tri-O-acetyl-β-D-galactopyranoside plays a crucial role in advancing our understanding of glycosidase function, glycosylation pathways, and carbohydrate biochemistry.