p-Nitrophenyl-2,3,4,6-tetra-O-acetyl-α-D-glucopyranoside (CAS 14131-42-1)

p-Nitrophenyl-2,3,4,6-tetra-O-acetyl-α-D-glucopyranoside is a substrate may be used in enzymatic assays to measure the activity of glycosidases. When added to a reaction mixture, p-Nitrophenyl-2,3,4,6-tetra-O-acetyl-α-D-glucopyranoside is hydrolyzed by glycosidases, resulting in the release of p-nitrophenol. The release of p-nitrophenol can be quantified spectrophotometrically at 400 nm, allowing for the determination of glycosidase activity. P-Nitrophenyl-2,3,4,6-Tetra-O-Acetyl-Α-D-Glucopyranoside is particularly useful in the study of glycosidase enzymes due to its specific substrate properties and the ease with which its hydrolysis product can be measured. Its mechanism of action involves serving as a substrate for glycosidases, leading to the release of a detectable product that can be used to quantify enzyme activity. P-Nitrophenyl-2,3,4,6-Tetra-O-Acetyl-Α-D-Glucopyranoside′s function in enzymatic assays is to provide a specific substrate for glycosidases, enabling the measurement of their activity in research and development applications.