p-Nitophenyl 4-O-(2,3,4,6-Tetra-O-acetyl-β-D-galactopyranosyl)- 2,3,6-tri-O-acetyl-β-D-glucopyranoside (CAS 84034-75-3)

p-Nitophenyl 4-O-(2,3,4,6-Tetra-O-acetyl-β-D-galactopyranosyl)-2,3,6-tri-O-acetyl-β-D-glucopyranoside is a synthetic compound widely utilized in biochemical and enzymatic research. Its chemical structure mimics natural glycosides, making it an excellent substrate analog for studying glycoside hydrolases and glycosyltransferases. The mechanism of action involves the enzymatic hydrolysis of the glycosidic bond by specific glycosidases, leading to the release of p-nitrophenol, which can be quantified spectrophotometrically due to its characteristic absorbance at a specific wavelength. This compound is particularly valuable in enzyme kinetics studies, substrate specificity assays, and inhibitor screening assays aimed at investigating the catalytic mechanisms of glycoside-modifying enzymes. Moreover, researchers have utilized this compound to explain the roles of glycosides in various biological processes, including carbohydrate metabolism and cell signaling pathways. Additionally, its chemical structure allows for modifications to design novel substrate analogs tailored to specific glycosidase targets, enabling researchers to probe enzyme-substrate interactions and develop potential interventions for glycoside-related disorders. Overall, p-Nitophenyl 4-O-(2,3,4,6-Tetra-O-acetyl-β-D-galactopyranosyl)-2,3,6-tri-O-acetyl-β-D-glucopyranoside continues to be a valuable tool in advancing our understanding of glycoside biology and enzyme function.