Date published: 2026-2-22

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p-MEK-1 Antibody (47.Ser 222): sc-136542

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Datasheets
  • p-MEK-1 Antibody (47.Ser 222) is a mouse monoclonal IgA, cited in 4 publications, provided at 200 µg/ml
  • raised against a short amino acid sequence containing phosphorylated Ser 222 of MEK-1 of human origin
  • recommended for detection of Ser 222 phosphorylated MEK-1 and Ser 222 phosphorylated MEK-2 of mouse, rat and human origin by WB, IP and ELISA
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-MEK-1 Antibody (47.Ser 222). This work is in progress.
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p-MEK-1 Antibody (47.Ser 222) is a mouse monoclonal IgA antibody that detects p-MEK-1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). p-MEK-1 (47.Ser 222) antibody is available in a non-conjugated form and specifically recognizes Ser 222 phosphorylated MEK-1 and MEK-2, which are critical components of the MAP kinase signaling pathway. MEK-1, also known as MAP2K1, plays a vital role in cellular signaling by acting as a dual-specificity kinase that phosphorylates both threonine and tyrosine residues on downstream targets, particularly extracellular signal-regulated kinases (ERKs). This phosphorylation activates ERKs, which drive various cellular processes, including proliferation, differentiation, and survival. Precise regulation of MEK-1 activity through phosphorylation is crucial, as dysregulation can lead to oncogenic transformations and contribute to various diseases, including cancer. Understanding p-MEK-1 function and regulation is important for developing targeted therapies and advancing knowledge of signal transduction mechanisms.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

p-MEK-1 Antibody (47.Ser 222)

sc-136542
200 µg/ml
$322.00

What is the recommended starting dilution for Western blot for p-MEK-1 (47.Ser 222): sc-136542 monoclonal antibody?

Asked by: jerojero
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-136542.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by from Not goodI tested it in our Hela cell lysate, and it did not work at all
Date published: 2018-04-03
Rated 4 out of 5 by from Working good!I got this for the free sample trial, and treated on B16 cells. It worked well.
Date published: 2018-03-07
Rated 1 out of 5 by from Didnot work for WBdid not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from Publishable Western blot results using humanPublishable Western blot results using human osteosarcoma cell lines (U2OS and HOS). -SCBT Publication Review
Date published: 2015-01-23
Rated 5 out of 5 by from Good WB publishable data gathered with humanGood WB publishable data gathered with human primary osteoblasts and Synovial fibroblasts. -SCBT Publication Review
Date published: 2014-05-24
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p-MEK-1 Antibody (47.Ser 222) is rated 3.2 out of 5 by 5.
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