p-KV4.2 Antibody (A-5): sc-377545

p-KV4.2 Antibody (A-5) is a mouse monoclonal IgM antibody that detects the Thr 607 phosphorylated KV4.2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). p-KV4.2 (A-5) antibody is available in a non-conjugated form, providing flexibility for various experimental needs. KV4.2 plays a crucial role in regulating neuronal excitability and action potential repolarization, which is essential for proper neuronal signaling and muscle contraction. Located primarily in the plasma membrane of excitable cells, KV4.2 channels are integral to the function of the central nervous system and the heart, where KV4.2 helps modulate the frequency and duration of action potentials. The assembly of KV4.2 into functional channels involves interactions with other KV subunits, which can influence channel gating kinetics and pharmacological properties. KV4.2 undergoes various post-translational modifications, including phosphorylation, which can significantly affect activity and interactions with other proteins. The phosphorylation of KV4.2 at specific residues, such as Thr 607, by kinases like ERK2, is critical for function and regulation, highlighting the importance of these modifications in cellular signaling pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-KV4.2 Antibody (A-5) References:

1. Subunit composition of Kv1 channels in human CNS.  |  Coleman, SK., et al. 1999. J Neurochem. 73: 849-58. PMID: 10428084
2. Kv4.2 phosphorylation by cyclic AMP-dependent protein kinase.  |  Anderson, AE., et al. 2000. J Biol Chem. 275: 5337-46. PMID: 10681507
3. Subunit composition determines Kv1 potassium channel surface expression.  |  Manganas, LN. and Trimmer, JS. 2000. J Biol Chem. 275: 29685-93. PMID: 10896669
4. Input-specific immunolocalization of differentially phosphorylated Kv4.2 in the mouse brain.  |  Varga, AW., et al. 2000. Learn Mem. 7: 321-32. PMID: 11040264
5. The A-type potassium channel Kv4.2 is a substrate for the mitogen-activated protein kinase ERK.  |  Adams, JP., et al. 2000. J Neurochem. 75: 2277-87. PMID: 11080179
6. Altered phosphorylation and localization of the A-type channel, Kv4.2 in status epilepticus.  |  Lugo, JN., et al. 2008. J Neurochem. 106: 1929-40. PMID: 18513371
7. Kv4.2 is a locus for PKC and ERK/MAPK cross-talk.  |  Schrader, LA., et al. 2009. Biochem J. 417: 705-15. PMID: 18795890
8. FMRP Mediates Chronic Ethanol-Induced Changes in NMDA, Kv4.2, and KChIP3 Expression in the Hippocampus.  |  Spencer, KB., et al. 2016. Alcohol Clin Exp Res. 40: 1251-61. PMID: 27147118
9. The brain Kv1.1 potassium channel: in vitro and in vivo studies on subunit assembly and posttranslational processing.  |  Deal, KK., et al. 1994. J Neurosci. 14: 1666-76. PMID: 8126562
10. Immunohistochemical localization of five members of the Kv1 channel subunits: contrasting subcellular locations and neuron-specific co-localizations in rat brain.  |  Veh, RW., et al. 1995. Eur J Neurosci. 7: 2189-205. PMID: 8563969
11. Beta subunits promote K+ channel surface expression through effects early in biosynthesis.  |  Shi, G., et al. 1996. Neuron. 16: 843-52. PMID: 8608002
12. Association and colocalization of the Kvbeta1 and Kvbeta2 beta-subunits with Kv1 alpha-subunits in mammalian brain K+ channel complexes.  |  Rhodes, KJ., et al. 1997. J Neurosci. 17: 8246-58. PMID: 9334400

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-KV4.2 Antibody (A-5) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-p-KV4.2 Antibody (A-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-KV4.2 Antibody (A-5) Specifications

Alternate Product Name: KV4.2

Product Clone Name: A-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: Thr 607

Epitope Target: KV4.2

Epitope Species: human

Product Reactivity/Detection: Thr 607 phosphorylated KV4.2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for p-KV4.2 Antibody (A-5): P: Blocking Peptide

The preferred KV4.2 antibody is KV4.2 Antibody (H-5): sc-390571.