p-Src Antibody (H-3): sc-166860

p-c-Src Antibody (H-3) is a mouse monoclonal IgG2b kappa light chain antibody raised against the pY530 site of human c-Src and pY535 site of mouse c-Src, with corresponding recognition of rat pY530, a critical regulatory modification of this tyrosine kinase. p-c-Src mouse monoclonal antibody (H-3) recognizes c-Src only when these specific tyrosine residues are phosphorylated and demonstrates effectiveness in Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry on paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications. Anti-p-c-Src monoclonal antibody (H-3) is available in non-conjugated form and multiple conjugated forms including agarose (Ag), horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® (AF) conjugates, offering versatility for diverse experimental needs. As a member of the Src family kinases, c-Src primarily resides in the cytoplasm but can undergo translocation to the plasma membrane in response to various stimuli. This dynamic localization enables c-Src to interact with membrane-associated proteins and participate in signaling cascades that regulate cellular responses to growth factors and other external signals. c-Src typically maintains an inactive state until specific cellular events, such as mitosis, trigger c-Src activation, highlighting c-Src′s importance in cell cycle progression and other dynamic cellular processes. Through c-Src′s broad expression pattern, particularly in neuronal tissues and platelets, c-Src plays crucial roles in neuronal differentiation and mature neuronal function maintenance. Through these diverse cellular functions, p-c-Src (H-3) antibody serves as an essential tool for researchers investigating signaling pathways that control cell proliferation, differentiation, and motility.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-Src Antibody (H-3) References:

1. Herpesvirus saimiri protein StpB associates with cellular Src.  |  Hör, S., et al. 2001. J Gen Virol. 82: 339-344. PMID: 11161272
2. Regulation of the oncogenic activity of the cellular src protein requires the correct spacing between the kinase domain and the C-terminal phosphorylated tyrosine (Tyr-527).  |  Cobb, BS., et al. 1991. Mol Cell Biol. 11: 5832-8. PMID: 1719372
3. Phosphorylated c-Src in the nucleus is associated with improved patient outcome in ER-positive breast cancer.  |  Campbell, EJ., et al. 2008. Br J Cancer. 99: 1769-74. PMID: 19018258
4. Phosphorylated c-Src is a novel predictor for recurrence in cervical squamous cell cancer patients.  |  Hou, T., et al. 2013. Int J Clin Exp Pathol. 6: 1121-7. PMID: 23696930
5. The cellular src gene product regulates junctional cell-to-cell communication.  |  Azarnia, R., et al. 1988. Science. 239: 398-401. PMID: 2447651
6. Sphingosine kinase 2 prevents the nuclear translocation of sphingosine 1-phosphate receptor-2 and tyrosine 416 phosphorylated c-Src and increases estrogen receptor negative MDA-MB-231 breast cancer cell growth: The role of sphingosine 1-phosphate receptor-4.  |  Ohotski, J., et al. 2014. Cell Signal. 26: 1040-7. PMID: 24486401
7. Extracellular Vesicle Activation of Latent HIV-1 Is Driven by EV-Associated c-Src and Cellular SRC-1 via the PI3K/AKT/mTOR Pathway.  |  Barclay, RA., et al. 2020. Viruses. 12: PMID: 32575590
8. Phospholipase C-gamma 1 associates with viral and cellular src kinases.  |  Nakanishi, O., et al. 1993. J Biol Chem. 268: 10754-9. PMID: 8388373
9. Association of tyrosine-phosphorylated c-Src with the cytoskeleton of hypertrophying myocardium.  |  Kuppuswamy, D., et al. 1997. J Biol Chem. 272: 4500-8. PMID: 9020175
10. Genetic variation of herpesvirus saimiri subgroup A transforming protein and its association with cellular src.  |  Lee, H., et al. 1997. J Virol. 71: 3817-25. PMID: 9094657

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Src Antibody (H-3) has 26 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Src Antibody (H-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Src Antibody (H-3) Specifications

Alternate Product Name: SRC

Product Clone Name: H-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: Tyr 530

Epitope Target: c-Src

Epitope Species: human

Product Reactivity/Detection: Tyr 530 phosphorylated c-Src of human and rat origin, and Tyr 535 phosphorylated c-Src of mouse origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 26 citations in various scientific publications

Available Conjugates/Forms for p-Src Antibody (H-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred Src antibody is Src Antibody (B-12): sc-8056.