p-Src Antibody (9A6): sc-81521

p-c-Src Antibody (9A6) is a mouse monoclonal IgG3 antibody raised against the Tyr419 phosphorylation site of human c-Src and Tyr424 phosphorylation site of mouse c-Src, with corresponding recognition of the rat homolog. p-c-Src mouse monoclonal antibody (9A6) recognizes c-Src only when these specific tyrosine residues are phosphorylated and demonstrates effectiveness in Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM) applications. Anti-p-c-Src monoclonal antibody (9A6) is available in non-conjugated form and serves as a specific tool for detecting activated c-Src kinase. c-Src′s structural organization is crucial for phosphorylation-dependent regulation, featuring an amino-terminal membrane anchor followed by SH3 and SH2 domains that facilitate protein-protein interactions and signal transduction. These structural elements enable c-Src to maintain an inactive state until specific cellular events, such as mitosis, trigger c-Src activation through phosphorylation. As a member of the Src family kinases, c-Src regulates essential cellular processes including proliferation, differentiation, motility, and adhesion. c-Src shows particularly high expression in neuronal tissues and platelets, highlighting c-Src′s significance in neuronal differentiation and mature neuronal cell function maintenance. Through these diverse roles and regulated activation states, c-Src serves as a critical mediator of cellular signaling pathways, making p-c-Src (9A6) antibody valuable for studying both normal cellular processes and disease mechanisms, particularly in cancer research.

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p-Src Antibody (9A6) References:

1. SRC family kinases mediate epithelial Na+ channel inhibition by endothelin.  |  Gilmore, ES., et al. 2001. J Biol Chem. 276: 42610-7. PMID: 11560932
2. src-related tyrosine protein kinases as signaling components in hematopoietic cells.  |  Eiseman, E. and Bolen, JB. 1990. Cancer Cells. 2: 303-10. PMID: 1704247
3. Phosphorylated c-Src in the nucleus is associated with improved patient outcome in ER-positive breast cancer.  |  Campbell, EJ., et al. 2008. Br J Cancer. 99: 1769-74. PMID: 19018258
4. Expression and interactions of the Src family of tyrosine protein kinases in T lymphocytes.  |  Bolen, JB., et al. 1991. Adv Cancer Res. 57: 103-49. PMID: 1950702
5. Neurones express high levels of a structurally modified, activated form of pp60c-src.  |  Brugge, JS., et al. Nature. 316: 554-7. PMID: 2412121
6. Blood platelets express high levels of the pp60c-src-specific tyrosine kinase activity.  |  Golden, A., et al. 1986. Proc Natl Acad Sci U S A. 83: 852-6. PMID: 2419901
7. Alterations in pp60c-src accompany differentiation of neurons from rat embryo striatum.  |  Cartwright, CA., et al. 1987. Mol Cell Biol. 7: 1830-40. PMID: 2439897
8. Developmental expression of two forms of pp60c-src in mouse brain.  |  Wiestler, OD. and Walter, G. 1988. Mol Cell Biol. 8: 502-4. PMID: 2447488
9. Genetic organization of human proto-oncogenes.  |  Sakaguchi, AY. 1983. Prog Clin Biol Res. 119: 93-103. PMID: 6191340
10. The PDGF receptor phosphorylates Tyr 138 in the c-Src SH3 domain in vivo reducing peptide ligand binding.  |  Broome, MA. and Hunter, T. 1997. Oncogene. 14: 17-34. PMID: 9010229

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Src Antibody (9A6) has 36 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Src Antibody (9A6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Src Antibody (9A6) Specifications

Alternate Product Name: SRC

Product Clone Name: 9A6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃

Amino Acids: Tyr 419

Epitope Target: c-Src

Epitope Species: human

Product Reactivity/Detection: Tyr 419 phosphorylated c-Src of human origin and Tyr 424 phosphorylated c-Src of mouse origin and the corresponding rat homolog

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 50 µg IgG₃ in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin, PEG and sucrose

Research Citations: 36 citations in various scientific publications

Available Conjugates/Forms for p-Src Antibody (9A6): N/A

The preferred Src antibody is Src Antibody (B-12): sc-8056.