p-β-dystroglycan Antibody (pY892.27.1): sc-136456

p-β-dystroglycan Antibody (pY892.27.1) is a mouse monoclonal IgG1 kappa light chain antibody that detects the phosphorylated β-dystroglycan protein of human, mouse, and rat origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). anti-p-β-dystroglycan antibody (pY892.27.1) is available in both non-conjugated and various conjugated forms, including agarose and horseradish peroxidase (HRP). Dystroglycan (DG) plays a crucial role as a cell surface receptor for several extracellular matrix components, such as laminins, Agrin, and Perlecan, which are essential for the formation of basement membranes during early development and the organization of laminin on the cell surface. DG′s ability to interact with these extracellular matrix molecules is vital for maintaining tissue integrity and facilitating cell signaling pathways. Additionally, DG forms a complex with dystrophin at the sarcolemma, contributing to the dystrophin-associated protein complex, and with utrophin at the neuromuscular junction, forming the utrophin-associated protein complex. This interaction is significant for the clustering of nicotinic acetylcholine receptors, which is essential for effective neuromuscular transmission. The phosphorylation of β-dystroglycan at Tyr 892 is particularly important for modulating its interactions and functions, making p-β-dystroglycan (pY892.27.1) monoclonal antibody a valuable tool for studying the dynamics of dystroglycan in various biological contexts.

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p-β-dystroglycan Antibody (pY892.27.1) References:

1. Chimaeric mice deficient in dystroglycans develop muscular dystrophy and have disrupted myoneural synapses.  |  Côté, PD., et al. 1999. Nat Genet. 23: 338-42. PMID: 10610181
2. Adhesion-dependent tyrosine phosphorylation of (beta)-dystroglycan regulates its interaction with utrophin.  |  James, M., et al. 2000. J Cell Sci. 113 (Pt 10): 1717-26. PMID: 10769203
3. Differential targeting of components of the dystrophin complex to the postsynaptic membrane.  |  Marchand, S., et al. 2001. Eur J Neurosci. 13: 221-9. PMID: 11168526
4. Distinct roles for dystroglycan, beta1 integrin and perlecan in cell surface laminin organization.  |  Henry, MD., et al. 2001. J Cell Sci. 114: 1137-44. PMID: 11228157
5. Expression of dystroglycan complex in satellite cells of dorsal root ganglia.  |  Masaki, T., et al. 2001. Acta Neuropathol. 101: 174-8. PMID: 11271373
6. Syntheses of alpha-dystroglycan derived glycosyl amino acids carrying a novel mannosyl serine/threonine linkage.  |  Seifert, J., et al. 2000. Glycoconj J. 17: 407-23. PMID: 11294507
7. The interaction of dystrophin with beta-dystroglycan is regulated by tyrosine phosphorylation.  |  Ilsley, JL., et al. 2001. Cell Signal. 13: 625-32. PMID: 11495720
8. Tyrosine phosphorylation of beta-dystroglycan at its WW domain binding motif, PPxY, recruits SH2 domain containing proteins.  |  Sotgia, F., et al. 2001. Biochemistry. 40: 14585-92. PMID: 11724572
9. Localization of phospho-beta-dystroglycan (pY892) to an intracellular vesicular compartment in cultured cells and skeletal muscle fibers in vivo.  |  Sotgia, F., et al. 2003. Biochemistry. 42: 7110-23. PMID: 12795607
10. Characterization of an Importin alpha/beta-recognized nuclear localization signal in beta-dystroglycan.  |  Lara-Chacón, B., et al. 2010. J Cell Biochem. 110: 706-17. PMID: 20512930
11. Retrograde trafficking of β-dystroglycan from the plasma membrane to the nucleus.  |  Gracida-Jiménez, V., et al. 2017. Sci Rep. 7: 9906. PMID: 28852008
12. Tyrosine phosphorylation as a regulator of dystrophin and beta-dystroglycan interaction: A molecular insight.  |  Abdullah, M., et al. 2020. J Mol Graph Model. 99: 107623. PMID: 32348939

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-β-dystroglycan Antibody (pY892.27.1) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-p-β-dystroglycan Antibody (pY892.27.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-β-dystroglycan Antibody (pY892.27.1) Specifications

Alternate Product Name: p-Dystroglycan

Product Clone Name: pY892.27.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Tyr 892

Epitope Target: β-dystroglycan

Epitope Species: human

Product Reactivity/Detection: Tyr 892 phosphorylated β-dystroglycan of human origin and correspondingly Tyr 890 phosphorylated β-dystroglycan of mouse origin and the corresponding rat homolog

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for p-β-dystroglycan Antibody (pY892.27.1): AC: Agarose, HRP: Horseradish Peroxidase

The preferred β-dystroglycan antibody is β-dystroglycan Antibody (4F7): sc-33702.