p-Bcl-2 Antibody (C-2): sc-377576

p-Bcl-2 Antibody (C-2) is a mouse monoclonal IgM antibody that detects Ser 87 phosphorylated Bcl-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). p-Bcl-2 (C-2) antibody is offered in a non-conjugated form, allowing for versatile experimental design. Bcl-2 plays a critical role in regulating apoptosis, a vital process that ensures proper cellular turnover and tissue homeostasis. Bcl-2 is primarily located in the outer mitochondrial membrane, where anti-Bcl-2 antibody (C-2) can detect its function in inhibiting pro-apoptotic factor release and preventing cell death. This localization allows Bcl-2 to effectively interact with other Bcl-2 family members, influencing the balance between survival and apoptosis. Through maintaining mitochondrial integrity, Bcl-2 helps regulate the cytochrome c/Apaf-1 pathway, essential for caspase activation and apoptosis execution. Dysregulation of Bcl-2, often due to mutations or aberrant expression, can lead to various cancers by allowing cells to evade programmed cell death, highlighting Bcl-2′s importance in both normal physiology and disease states.

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p-Bcl-2 Antibody (C-2) References:

1. Dephosphorylation targets Bcl-2 for ubiquitin-dependent degradation: a link between the apoptosome and the proteasome pathway.  |  Dimmeler, S., et al. 1999. J Exp Med. 189: 1815-22. PMID: 10359585
2. The apoptotic v-cyclin-CDK6 complex phosphorylates and inactivates Bcl-2.  |  Ojala, PM., et al. 2000. Nat Cell Biol. 2: 819-25. PMID: 11056537
3. Bcl-2 family proteins as targets for anticancer drug design.  |  Huang, Z. 2000. Oncogene. 19: 6627-31. PMID: 11426648
4. Overexpressed full-length human BCL2 extends the survival of baculovirus-infected Sf9 insect cells.  |  Alnemri, ES., et al. 1992. Proc Natl Acad Sci U S A. 89: 7295-9. PMID: 1502141
5. Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death.  |  Hockenbery, D., et al. 1990. Nature. 348: 334-6. PMID: 2250705
6. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics.  |  Kerr, JF., et al. 1972. Br J Cancer. 26: 239-57. PMID: 4561027
7. Inactivation of Bcl-2 by phosphorylation.  |  Haldar, S., et al. 1995. Proc Natl Acad Sci U S A. 92: 4507-11. PMID: 7753834
8. Bcl-2 and the regulation of programmed cell death.  |  Reed, JC. 1994. J Cell Biol. 124: 1-6. PMID: 8294493
9. Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked.  |  Yang, J., et al. 1997. Science. 275: 1129-32. PMID: 9027314
10. The Bcl-2 protein family: arbiters of cell survival.  |  Adams, JM. and Cory, S. 1998. Science. 281: 1322-6. PMID: 9735050

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Bcl-2 Antibody (C-2) has 9 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Bcl-2 Antibody (C-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Bcl-2 Antibody (C-2) Specifications

Alternate Product Name: Bcl-2

Product Clone Name: C-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: Ser 87

Epitope Target: Bcl-2

Epitope Species: human

Product Reactivity/Detection: Ser 87 phosphorylated Bcl-2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 9 citations in various scientific publications

Available Conjugates/Forms for p-Bcl-2 Antibody (C-2): P: Blocking Peptide

The preferred Bcl-2 antibody is Bcl-2 Antibody (C-2): sc-7382.