Molecular structure of p-Azidophenacyl Bromide, CAS Number: 57018-46-9
p-Azidophenacyl Bromide (CAS 57018-46-9)
Alternate Names:
1-(4-Azidophenyl)-2-bromo-ethanone
Application:
p-Azidophenacyl Bromide is an aryl azide-based, sulfhydryl- and photoreactive crosslinker
CAS Number:
57018-46-9
Molecular Weight:
240.06
Molecular Formula:
C8H6BrN3O
Supplemental Information:
This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
QUICK LINKS
Ordering Information
Description
Technical Information
Safety Information
SDS & Certificate of Analysis
p-Azidophenacyl Bromide is a chemical photoreactive crosslinker. It is in the study of protein-protein interactions and protein structure. When exposed to ultraviolet light, p-Azidophenacyl Bromide undergoes a photochemical reaction, forming a highly reactive nitrene intermediate. This nitrene can then covalently crosslink with nearby amino acid residues in proteins, allowing for the study of protein complexes and protein-protein interactions. The use of p-Azidophenacyl Bromide as a crosslinker in biochemistry has been well-documented and be effective in various experimental applications. Its ability to selectively crosslink proteins under controlled conditions may be useful for studying the structure and function of proteins in biological systems.
p-Azidophenacyl Bromide (CAS 57018-46-9) References
- The second stalk of the yeast ATP synthase complex: identification of subunits showing cross-links with known positions of subunit 4 (subunit b). | Soubannier, V., et al. 1999. Biochemistry. 38: 15017-24. PMID: 10555984
- Probing the structure of the PI-SceI-DNA complex by affinity cleavage and affinity photocross-linking. | Hu, D., et al. 2000. J Biol Chem. 275: 2705-12. PMID: 10644733
- Probing contacts between the ribonuclease H domain of HIV-1 reverse transcriptase and nucleic acid by site-specific photocross-linking. | Rausch, JW., et al. 2000. J Biol Chem. 275: 16015-22. PMID: 10748161
- P-Azidophenacyl bromide, a versatile photolabile bifunctional reagent. Reaction with glyceraldehyde-3-phosphate dehydrogenase. | Hixson, SH. and Hixson, SS. 1975. Biochemistry. 14: 4251-4. PMID: 1237309
- Structural characterization of phospholamban in cardiac sarcoplasmic reticulum membranes by cross-linking. | Young, EF., et al. 1989. Membr Biochem. 8: 95-106. PMID: 2634236
- Site-directed cross-linking of mRNA analogues to the Escherichia coli ribosome; identification of 30S ribosomal components that can be cross-linked to the mRNA at various points 5' with respect to the decoding site. | Stade, K., et al. 1989. Nucleic Acids Res. 17: 9889-908. PMID: 2690012
- YpeB dimerization may be required to stabilize SleB for effective germination of Bacillus anthracis spores. | Sayer, CV. and Popham, DL. 2019. BMC Microbiol. 19: 169. PMID: 31349814
- Site-specific DNA Mapping of Protein Binding Orientation Using Azidophenacyl Bromide (APB). | Perera, HM. and Trakselis, MA. 2020. Bio Protoc. 10: e3649. PMID: 33659320
- Photochemical cross-linking of unmodified acetylvalyl-tRNA to 16S RNA at the ribosomal P site. | Schwartz, I. and Ofengand, J. 1978. Biochemistry. 17: 2524-30. PMID: 354688
- Preparation of probe-modified RNA with 5-mercapto-UTP for analysis of protein-RNA interactions. | He, B., et al. 1995. Nucleic Acids Res. 23: 1231-8. PMID: 7537876
- Photoaffinity cross-linking of TaqI restriction endonuclease using an aryl azide linked to the phosphate backbone. | Mayer, AN. and Barany, F. 1995. Gene. 153: 1-8. PMID: 7883172
- Orientation of the LexA DNA-binding motif on operator DNA as inferred from cysteine-mediated phenyl azide crosslinking. | Dumoulin, P., et al. 1993. Proc Natl Acad Sci U S A. 90: 2030-4. PMID: 8446625
- P-azidoiodoacetanilide, a new short photocrosslinker that has greater cysteine specificity than p-azidophenacyl bromide and p-azidobromoacetanilide. | Zhang, J., et al. 1995. Biochem Biophys Res Commun. 217: 1177-84. PMID: 8554574
- Structure of the LexA repressor-DNA complex probed by affinity cleavage and affinity photo-cross-linking. | Dumoulin, P., et al. 1996. Biochemistry. 35: 4279-86. PMID: 8605176
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-Azidophenacyl Bromide, 1 g | sc-212500 | 1 g | $700.00 |