p-Adenovirus-2 E1A Antibody (C-9): sc-374663

p-Adenovirus-2 E1A Antibody (C-9) is a mouse monoclonal IgG1 antibody that detects Adenovirus-2 E1A by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Adenovirus-2 E1A antibody (C-9) plays a crucial role in studying the early region 1A (E1A) of the adenovirus genome, which is essential for understanding its transforming activity. Adenovirus-2 E1A (C-9) monoclonal antibody functions primarily as a tool for detecting E1A protein, which acts as a transcriptional regulator, influencing DNA synthesis and promoting cell transformation, significant in viral oncogenesis. Located in the nucleus, E1A interacts with various cellular proteins, including tumor suppressor p105/Rb-1 and cyclin A, to modulate cell cycle progression and transcriptional activity. E1A proteins undergo post-translational modifications, such as phosphorylation at key serine residues, which can impact their function and interactions. Phosphorylation at Ser 89 and Ser 219 is critical for E1A′s transforming activity, with Ser 89 phosphorylation influencing the transformation of primary rat kidney cells. Anti-Adenovirus-2 E1A antibody (C-9) detection of these modifications makes this reagent invaluable for researchers investigating molecular mechanisms of adenoviral transformation and implications in cancer biology.

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p-Adenovirus-2 E1A Antibody (C-9) References:

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2. The interaction of RNA polymerase II with the adenovirus-2 major late promoter is precluded by phosphorylation of the C-terminal domain of subunit IIa.  |  Chesnut, JD., et al. 1992. J Biol Chem. 267: 10500-6. PMID: 1316903
3. Enzymatic activities associated with a purified simian virus 40 T antigen-related protein.  |  Tjian, R. and Robbins, A. 1979. Proc Natl Acad Sci U S A. 76: 610-4. PMID: 218212
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5. Poor growth of human adenovirus-12 compared to adenovirus-2 correlates with a failure to impair PKR activation during the late phase of infection.  |  Wu, C., et al. 2015. Virology. 475: 120-8. PMID: 25462352
6. The transition of RNA polymerase II from initiation to elongation is associated with phosphorylation of the carboxyl-terminal domain of subunit IIa.  |  Payne, JM., et al. 1989. J Biol Chem. 264: 19621-9. PMID: 2584185
7. The major late promoter of adenovirus-2 is accurately transcribed by RNA polymerases IIO, IIA, and IIB.  |  Kim, WY. and Dahmus, ME. 1989. J Biol Chem. 264: 3169-76. PMID: 2914948
8. Biosynthesis and properties of the adenovirus 2 L1-encoded 52,000- and 55,000-Mr proteins.  |  Lucher, LA., et al. 1986. J Virol. 57: 839-47. PMID: 3005630
9. RNA polymerases IIA and IIO have distinct roles during transcription from the TATA-less murine dihydrofolate reductase promoter.  |  Kang, ME. and Dahmus, ME. 1993. J Biol Chem. 268: 25033-40. PMID: 8227067
10. A region in the C-terminus of adenovirus 2/5 E1a protein is required for association with a cellular phosphoprotein and important for the negative modulation of T24-ras mediated transformation, tumorigenesis and metastasis.  |  Boyd, JM., et al. 1993. EMBO J. 12: 469-78. PMID: 8440238

The preferred Adenovirus-2/5 E1A antibody is Adenovirus-2/5 E1A Antibody (M73): sc-25.