Ox40 Antibody (BER-Act35): sc-20073

Ox40 Antibody (BER-Act35) is a mouse monoclonal IgG1 kappa light chain antibody that detects human Ox40 by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-Ox40 antibody (BER-Act35) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Ox40, also known as CD134 or Ox40R, is a crucial member of the tumor necrosis factor receptor (TNFR) family, playing a significant role in immune response. Ox40 is primarily located on activated CD4+ T lymphocytes, where Ox40 (BER-Act35) antibody is essential for coordinating T cell selection, migration, and cytokine differentiation, particularly in T helper (Th)1 and Th2 cells. The interaction between Ox40 and Ox40L, predominantly expressed on activated B cells, provides a vital costimulatory signal that enhances T cell activation and promotes robust CD4+ T cell responses both in vivo and in vitro. This signaling pathway regulates immune responses, influencing outcomes of infections, inflammation, autoimmune diseases, and maintaining tissue homeostasis. Anti-Ox40 antibody (BER-Act35) in various formats allows versatile applications in research, enabling scientists to explore Ox40′s intricate roles in immune regulation and therapeutic interventions.

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LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Ox40 Antibody (BER-Act35) References:

1. Ox40-ligand has a critical costimulatory role in dendritic cell:T cell interactions.  |  Chen, AI., et al. 1999. Immunity. 11: 689-98. PMID: 10626891
2. OX40-deficient mice are defective in Th cell proliferation but are competent in generating B cell and CTL Responses after virus infection.  |  Kopf, M., et al. 1999. Immunity. 11: 699-708. PMID: 10626892
3. Role of OX40 signals in coordinating CD4 T cell selection, migration, and cytokine differentiation in T helper (Th)1 and Th2 cells.  |  Lane, P. 2000. J Exp Med. 191: 201-6. PMID: 10637265
4. Impairment of antigen-presenting cell function in mice lacking expression of OX40 ligand.  |  Murata, K., et al. 2000. J Exp Med. 191: 365-74. PMID: 10637280
5. Engagement of the OX-40 receptor in vivo enhances antitumor immunity.  |  Weinberg, AD., et al. 2000. J Immunol. 164: 2160-9. PMID: 10657670
6. CD134L engagement enhances human B cell Ig production: CD154/CD40, CD70/CD27, and CD134/CD134L interactions coordinately regulate T cell-dependent B cell responses.  |  Morimoto, S., et al. 2000. J Immunol. 164: 4097-104. PMID: 10754303
7. Therapeutic strategies for the costimulatory molecule OX40 in T-cell-mediated immunity.  |  Fu, Y., et al. 2020. Acta Pharm Sin B. 10: 414-433. PMID: 32140389
8. OX40-OX40L Inhibition for the Treatment of Atopic Dermatitis-Focus on Rocatinlimab and Amlitelimab.  |  Lé, AM. and Torres, T. 2022. Pharmaceutics. 14: PMID: 36559247
9. Diverse Role of OX40 on T Cells as a Therapeutic Target for Skin Diseases.  |  Iriki, H., et al. 2023. J Invest Dermatol. 143: 545-553. PMID: 36842860
10. OX40 in the Pathogenesis of Atopic Dermatitis-A New Therapeutic Target.  |  Croft, M., et al. 2024. Am J Clin Dermatol. 25: 447-461. PMID: 38236520
11. The role of OX40 ligand/OX40 axis signalling in atopic dermatitis.  |  Guttman-Yassky, E., et al. 2024. Br J Dermatol. 191: 488-496. PMID: 38836560
12. The TNF receptor superfamily of cellular and viral proteins: activation, costimulation, and death.  |  Smith, CA., et al. 1994. Cell. 76: 959-62. PMID: 8137429

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Ox40 Antibody (BER-Act35) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-Ox40 Antibody (BER-Act35) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Ox40 Antibody (BER-Act35) Specifications

Alternate Product Name: Ox40

Product Clone Name: BER-Act35

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Ox40

Epitope Species: human

Product Reactivity/Detection: Ox40

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for Ox40 Antibody (BER-Act35): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin