Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
osteopontin/OPN/SPP1 Double Nickase Plasmid (h): sc-400219-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- osteopontin/OPN/SPP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- osteopontin/OPN/SPP1 Double Nickase Plasmid (h) and osteopontin/OPN/SPP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SPP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: osteopontin/OPN/SPP1 Antibody (AKm2A1): sc-21742
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
osteopontin/OPN/SPP1 Double Nickase Plasmid (h) | sc-400219-NIC | 20 µg | $410.00 | |||
osteopontin/OPN/SPP1 Double Nickase Plasmid (h2) | sc-400219-NIC-2 | 20 µg | $410.00 |
SPP1 encodes osteopontin (OPN), a secreted phosphoglycoprotein that also functions as an extracellular matrix-associated cytokine regulating cell adhesion, migration, and survival. OPN signals through integrins (for example αvβ3) and CD44 to modulate focal adhesion dynamics, cytoskeletal remodeling, and downstream pathways including PI3K–AKT, MAPK/ERK, and NF-κB. It is expressed by multiple cell types such as immune and stromal populations and contributes to inflammatory signaling, macrophage polarization, and tissue remodeling programs. Dysregulated SPP1/OPN expression is frequently studied in cancer invasion and metastasis, fibrosis, and chronic inflammatory conditions, where it serves as a mechanistic node linking extracellular cues to pro-migratory and pro-survival transcriptional responses.
osteopontin/OPN/SPP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SPP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SPP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SPP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SPP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.