osteocalcin Double Nickase Plasmid (h): sc-400299-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- osteocalcin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- osteocalcin Double Nickase Plasmid (h) and osteocalcin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting BGLAP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: osteocalcin Antibody (G-5): sc-365797
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
osteocalcin Double Nickase Plasmid (h) | sc-400299-NIC | 20 µg | $410.00 | |||
osteocalcin Double Nickase Plasmid (h2) | sc-400299-NIC-2 | 20 µg | $410.00 |
BGLAP encodes osteocalcin, a vitamin K–dependent, γ-carboxylated extracellular matrix protein secreted by osteoblasts and incorporated into hydroxyapatite during bone mineralization. Osteocalcin participates in osteoblast differentiation programs and bone remodeling processes coordinated with RUNX2-driven transcription, collagen matrix deposition, and calcium/phosphate homeostasis. Altered BGLAP expression or osteocalcin maturation is frequently used as a readout of osteogenic state and has been associated with dysregulated bone turnover in conditions such as osteoporosis, osteomalacia, and tumor-associated osteolysis. As a matrix-associated marker, osteocalcin supports mechanistic studies of skeletal development, mineralization dynamics, and microenvironmental signaling in bone biology models.
osteocalcin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the BGLAP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within BGLAP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt BGLAP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of BGLAP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.