Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
osteocalcin CRISPR Activation Plasmid (h): sc-400299-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- osteocalcin CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- osteocalcin CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by osteocalcin CRISPR Activation Plasmid (h) and osteocalcin CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the BGLAP transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: osteocalcin Antibody (G-5): sc-365797
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
osteocalcin CRISPR Activation Plasmid (h) | sc-400299-ACT | 20 µg | $397.00 | |||
osteocalcin CRISPR Activation Plasmid (h2) | sc-400299-ACT-2 | 20 µg | $397.00 |
BGLAP encodes osteocalcin, a vitamin K–dependent, γ-carboxylated extracellular matrix protein secreted by osteoblasts and incorporated into the mineralized bone matrix. Osteocalcin participates in bone formation and remodeling by influencing hydroxyapatite deposition, mineral maturation, and osteoblast–osteoclast coupling, and is commonly used as a marker of osteogenic differentiation. Its expression is regulated by osteogenic signaling networks including RUNX2-driven transcriptional programs and pathways linked to mineral metabolism. Dysregulated BGLAP/osteocalcin expression or processing is associated with altered bone turnover states and provides a functional readout in studies of skeletal development and musculoskeletal disease mechanisms.
osteocalcin CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous BGLAP expression without altering the underlying DNA sequence.
osteocalcin CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the BGLAP locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the BGLAP transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous osteocalcin expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native BGLAP locus and enabling the study of osteocalcin-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of osteocalcin pathway restoration in tumor cells with silenced or reduced BGLAP expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.