OMP CRISPR Activation Plasmid (h): sc-403257-ACT

Human OMP (olfactory marker protein) encodes a small cytosolic protein highly enriched in mature olfactory sensory neurons, where it supports odor-evoked signal transduction and neuronal maturation. OMP modulates intracellular second-messenger dynamics, including cAMP- and Ca2+-dependent processes, influencing response kinetics and adaptation in the olfactory pathway. Altered OMP expression is widely used as a readout of olfactory neuron differentiation state and circuit integrity in studies of sensory neurobiology. Dysregulation of olfactory neuron function and maturation is relevant to investigations of hyposmia/anosmia and broader neurodevelopmental or neurodegenerative contexts where olfactory deficits can serve as a measurable phenotype.

OMP CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous OMP expression without altering the underlying DNA sequence.

OMP CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the OMP locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.

Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the OMP transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous OMP expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native OMP locus and enabling the study of OMP-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of OMP pathway restoration in tumor cells with silenced or reduced OMP expression.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.