Oct3/4 Antibody (C-10): sc-5279

Oct-3/4 Antibody (C-10) is a mouse monoclonal IgG2b kappa light chain antibody that detects Oct-3/4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Oct-3/4 monoclonal antibody (C-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Oct-3/4, also known as POU5F1, is referred to as octamer-binding transcription factor-3 (Oct-3 or Otf-3) and octamer-binding transcription factor-4 (Oct-4 or Otf-4), respectively. Oct-3/4 plays a crucial role in regulating embryonic stem cell populations by influencing lineage commitment and maintaining pluripotency. This transcription factor is essential for sustaining stem-cell self-renewal and guiding differentiation pathways, which are vital for proper embryonic development. Oct-3/4 activates the UTF1 gene, promoting rapid proliferation and tumorigenic properties in embryonic stem cells. In humans, two isoforms of Oct-3/4 are involved in maintaining the undifferentiated state of embryonic stem cells, while pseudogenes located on chromosomes 10 and 8 have been shown to be transcribed in certain cancer cell lines and tissues, highlighting Oct-3/4′s significance in both normal development and cancer biology. Anti-Oct-3/4 antibody (C-10) is an essential tool for researchers investigating Oct-3/4′s role in stem cell biology, development, and implications in tumorigenesis, providing valuable insights into both physiological and pathological processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Oct3/4 Antibody (C-10) References:

1. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells.  |  Niwa, H., et al. 2000. Nat Genet. 24: 372-6. PMID: 10742100
2. Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissues.  |  Takeda, J., et al. 1992. Nucleic Acids Res. 20: 4613-20. PMID: 1408763
3. Oct-3/4 maintains the proliferative embryonic stem cell state via specific binding to a variant octamer sequence in the regulatory region of the UTF1 locus.  |  Nishimoto, M., et al. 2005. Mol Cell Biol. 25: 5084-94. PMID: 15923625
4. Characterization of putative cis-regulatory elements that control the transcriptional activity of the human Oct4 promoter.  |  Yang, HM., et al. 2005. J Cell Biochem. 96: 821-30. PMID: 16149048
5. Oct4 pseudogenes are transcribed in cancers.  |  Suo, G., et al. 2005. Biochem Biophys Res Commun. 337: 1047-51. PMID: 16229821
6. Interaction between Oct3/4 and Cdx2 determines trophectoderm differentiation.  |  Niwa, H., et al. 2005. Cell. 123: 917-29. PMID: 16325584
7. Stem cell research. Four genes confer embryonic potential.  |  Vogel, G. 2006. Science. 313: 27. PMID: 16825541
8. POU5F1 isoforms show different expression patterns in human embryonic stem cells and preimplantation embryos.  |  Cauffman, G., et al. 2006. Stem Cells. 24: 2685-91. PMID: 16916925
9. Oct-3/4 promotes migration and invasion of glioblastoma cells.  |  Kobayashi, K., et al. 2012. J Cell Biochem. 113: 508-17. PMID: 21938739
10. Cellular Functions of OCT-3/4 Regulated by Ubiquitination in Proliferating Cells.  |  Baek, KH., et al. 2020. Cancers (Basel). 12: PMID: 32178477
11. Inducible expression of Oct-3/4 reveals synergy with Klf4 in targeting Cyclin A2 to enhance proliferation during early reprogramming.  |  Zaveri, L. and Dhawan, J. 2022. Biochem Biophys Res Commun. 587: 29-35. PMID: 34864392
12. Formation of pluripotent stem cells in the mammalian embryo depends on the POU transcription factor Oct4.  |  Nichols, J., et al. 1998. Cell. 95: 379-91. PMID: 9814708

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Oct3/4 Antibody (C-10) has 2,698 citations in a variety of scientific publications.

Primary antibodies like Anti-Oct3/4 Antibody (C-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Oct3/4 Antibody (C-10) Specifications

Alternate Product Name: OCT4, POU5F1

Product Clone Name: C-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-134

Epitope Target: Oct-3/4

Epitope Species: human

Product Reactivity/Detection: Oct-3/4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2,698 citations in various scientific publications

Available Conjugates/Forms for Oct3/4 Antibody (C-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent