Oct-2 Antibody (PT2): sc-56822
- Oct-2 Antibody (PT2) is a mouse monoclonal IgG1 κ Oct-2 antibody, cited in 4 publications, provided at 200 µg/ml
- raised against full length Oct-2 of human origin
- Oct-2 Antibody (PT2) is recommended for detection of Oct-2 of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-Oct-2 Antibody (PT2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-56822 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Oct-2 Antibody (PT2) for WB and IHC(P) applications. These reagents are now offered in bundles with Oct-2 Antibody (PT2) (see ordering information below).
Oct-2 Antibody (PT2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Oct-2 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-Oct-2 antibody (PT2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Oct-2, also known as POU class 2 homeobox 2 (POU2F2), plays a crucial role in regulating gene expression, particularly in B cells, where Oct-2 is essential for activating immunoglobulin gene promoters. Oct-2 (PT2) monoclonal antibody localizes to the nucleus, where Oct-2 binds to specific DNA sequences known as octamer motifs, which are vital for transcriptional regulation of genes involved in immune response. Oct-2′s interaction with other transcription factors and coactivators, such as OBF1, enhances regulatory capacity, making Oct-2 a key player in B lymphocyte development and function. Understanding Oct-2′s function and localization helps elucidate immune regulation mechanisms and develop potential therapeutic strategies targeting B cell-related disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Oct-2 Antibody (PT2) References:
- Synergism with the coactivator OBF-1 (OCA-B, BOB-1) is mediated by a specific POU dimer configuration. | Tomilin, A., et al. 2000. Cell. 103: 853-64. PMID: 11136971
- NF-kappa B and Oct-2 synergize to activate the human 3' Igh hs4 enhancer in B cells. | Sepulveda, MA., et al. 2004. J Immunol. 172: 1054-64. PMID: 14707079
- A human lymphoid-specific transcription factor that activates immunoglobulin genes is a homoeobox protein. | Scheidereit, C., et al. 1988. Nature. 336: 551-7. PMID: 2904654
- The B-cell-specific Oct-2 protein contains POU box- and homeo box-type domains. | Clerc, RG., et al. 1988. Genes Dev. 2: 1570-81. PMID: 3265124
- Regulation of solute carriers oct2 and OAT1/3 in the kidney: a phylogenetic, ontogenetic, and cell dynamic perspective. | Pou Casellas, C., et al. 2022. Physiol Rev. 102: 993-1024. PMID: 34486394
- Challenges and Opportunities for Improved Drug-Drug Interaction Predictions for Renal OCT2 and MATE1/2-K Transporters. | Krishnan, S., et al. 2022. Clin Pharmacol Ther. 112: 562-572. PMID: 35598119
- Deletion of the mRNA endonuclease Regnase-1 promotes NK cell anti-tumor activity via OCT2-dependent transcription of Ifng. | Sun, X., et al. 2024. Immunity. 57: 1360-1377.e13. PMID: 38821052
- The Oct-2 glutamine-rich and proline-rich activation domains can synergize with each other or duplicates of themselves to activate transcription. | Tanaka, M., et al. 1994. Mol Cell Biol. 14: 6046-55. PMID: 8065338
- The Oct-2 transcription factor. | Latchman, DS. 1996. Int J Biochem Cell Biol. 28: 1081-3. PMID: 8930131
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Oct-2 Antibody (PT2) | sc-56822 | 200 µg/ml | $316.00 | |||
Oct-2 Antibody (PT2): m-IgG Fc BP-HRP Bundle | sc-528621 | 200 µg Ab; 10 µg BP | $354.00 | |||
Oct-2 Antibody (PT2): m-IgGκ BP-HRP Bundle | sc-521045 | 200 µg Ab, 40 µg BP | $354.00 | |||
Oct-2 Antibody (PT2): m-IgG1 BP-HRP Bundle | sc-543033 | 200 µg Ab; 20 µg BP | $354.00 | |||
Oct-2 Antibody (PT2) X | sc-56822 X | 200 µg/0.1 ml | $316.00 | |||
Oct-2 Antibody (PT2) AC | sc-56822 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Oct-2 Antibody (PT2) HRP | sc-56822 HRP | 200 µg/ml | $316.00 | |||
Oct-2 Antibody (PT2) FITC | sc-56822 FITC | 200 µg/ml | $330.00 | |||
Oct-2 Antibody (PT2) PE | sc-56822 PE | 200 µg/ml | $343.00 | |||
Oct-2 Antibody (PT2) Alexa Fluor® 488 | sc-56822 AF488 | 200 µg/ml | $357.00 | |||
Oct-2 Antibody (PT2) Alexa Fluor® 546 | sc-56822 AF546 | 200 µg/ml | $357.00 | |||
Oct-2 Antibody (PT2) Alexa Fluor® 594 | sc-56822 AF594 | 200 µg/ml | $357.00 | |||
Oct-2 Antibody (PT2) Alexa Fluor® 647 | sc-56822 AF647 | 200 µg/ml | $357.00 | |||
Oct-2 Antibody (PT2) Alexa Fluor® 680 | sc-56822 AF680 | 200 µg/ml | $357.00 | |||
Oct-2 Antibody (PT2) Alexa Fluor® 790 | sc-56822 AF790 | 200 µg/ml | $357.00 |