Date published: 2025-9-5
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Ob Antibody (F-3) - Immunohistochemistry - Image 293336
Ob 抗体 (F-3): sc-48408. 对福尔马林固定, 石蜡包埋的人类卵巢组织进行免疫过氧化物酶染色, 显示内皮细胞的细胞质染色和血管中的血浆染色.
Ob 抗体 (F-3): sc-48408. 对福尔马林固定, 石蜡包埋的人类卵巢组织进行免疫过氧化物酶染色, 显示内皮细胞的细胞质染色和血管中的血浆染色.

Ob 抗体 (F-3): sc-48408

3.7(7)
寫評論提問

说明书
  • Ob 抗体 F-3 是小鼠单克隆 IgG2a κ，Ob抗体, 在4篇文献中引用，规格为200 µg/ml
  • 与human的92-145氨基酸残基的Ob
  • Ob 抗体 (F-3) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 Ob
  • 抗Ob抗体（F-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ob (F-3): sc-48408的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 Ob 抗体 (F-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Ob 抗体 (F-3) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Ob 抗体（F-3）是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过西部印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附试验（ELISA）检测人源 Ob 蛋白。抗 Ob 抗体 (F-3) 有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。Ob 蛋白又称瘦素，在通过抑制饥饿来调节能量平衡方面起着至关重要的作用，对维持体重平衡至关重要。瘦素主要由脂肪组织产生，作用于下丘脑中的受体，发出饱腹信号，从而影响食物摄入和能量消耗。瘦素信号传导失调与肥胖和代谢紊乱有关，因此 Ob 蛋白对于了解这些疾病的分子机制至关重要。Ob 基因编码一种 167 个氨基酸的蛋白质，该蛋白质在不同物种间高度保守，人类和小鼠序列之间的一致性高达 84%，凸显了进化的重要性。在最初的先天性 C57BL/6J Ob/Ob 小鼠品系中发现了 Ob 基因的无义突变，该品系是研究肥胖和相关代谢疾病的模型。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ob 抗体 (F-3) 参考文献:

  1. 瘦素与营养不良  |  Prentice, AM., et al. 2002. Nutr Rev. 60: S56-67; discussion S68-84, 85-7. PMID: 12403085
  2. 瘦素与心血管系统  |  Rahmouni, K. and Haynes, WG. 2004. Recent Prog Horm Res. 59: 225-44. PMID: 14749504
  3. 解决重大问题  |  Friedman, JM. and Leibel, RL. 1992. Cell. 69: 217-20. PMID: 1568242
  4. 小鼠 ob 突变的分子图谱。  |  Friedman, JM., et al. 1991. Genomics. 11: 1054-62. PMID: 1686014
  5. 瘦素与心血管疾病  |  Hou, N. and Luo, JD. 2011. Clin Exp Pharmacol Physiol. 38: 905-13. PMID: 21957899
  6. 瘦素对胶质母细胞瘤的促进作用  |  Lawrence, JE., et al. 2012. Neurol Res Int. 2012: 870807. PMID: 22263109
  7. 瘦素与认知  |  Warren, MW., et al. 2012. Dement Geriatr Cogn Disord. 33: 410-5. PMID: 22814193
  8. 瘦素与生殖功能  |  Hausman, GJ., et al. 2012. Biochimie. 94: 2075-81. PMID: 22980196
  9. 人类肥胖基因的结构组织和染色体分配。  |  Isse, N., et al. 1995. J Biol Chem. 270: 27728-33. PMID: 7499240
  10. 遗传学寻找饱腹感因子  |  Rink, TJ. 1994. Nature. 372: 406-7. PMID: 7984230
  11. 小鼠肥胖基因及其人类同源物的定位克隆。  |  Zhang, Y., et al. 1994. Nature. 372: 425-32. PMID: 7984236
  12. 肥胖症和高血压  |  El-Atat, F., et al. 2003. Endocrinol Metab Clin North Am. 32: 823-54. PMID: 14711064

订购信息

产品名称产品编号规格价格数量收藏夹

Ob 抗体 (F-3)

sc-48408
200 µg/ml
$316.00

Ob (F-3): m-IgG Fc BP-HRP 套装

sc-525563
200 µg Ab; 10 µg BP
$354.00

Ob (F-3): m-IgG2a BP-HRP 套装

sc-546316
200 µg Ab; 10 µg BP
$354.00

Ob 抗体 (F-3) AC

sc-48408 AC
500 µg/ml, 25% agarose
$416.00

Ob 抗体 (F-3) HRP

sc-48408 HRP
200 µg/ml
$316.00

Ob 抗体 (F-3) FITC

sc-48408 FITC
200 µg/ml
$330.00

Ob 抗体 (F-3) PE

sc-48408 PE
200 µg/ml
$343.00

Ob 抗体 (F-3) Alexa Fluor® 488

sc-48408 AF488
200 µg/ml
$357.00

Ob 抗体 (F-3) Alexa Fluor® 546

sc-48408 AF546
200 µg/ml
$357.00

Ob 抗体 (F-3) Alexa Fluor® 594

sc-48408 AF594
200 µg/ml
$357.00

Ob 抗体 (F-3) Alexa Fluor® 647

sc-48408 AF647
200 µg/ml
$357.00

Ob 抗体 (F-3) Alexa Fluor® 680

sc-48408 AF680
200 µg/ml
$357.00

Ob 抗体 (F-3) Alexa Fluor® 790

sc-48408 AF790
200 µg/ml
$357.00

Hi, I am wondering if the sc-48408 antibody has been used tested on formalin-fixed paraffin-embedded feline samples? What is the expected cross-reactivity between human and cat for the antibody?

Asked by: CeciliaL
Thank you for your question. We have not tested this antibody, Ob (F-3): sc-48408, on feline samples. And we are not aware of any other publications or references from other researchers using the antibody on feline samples. We are not aware of a published protein sequence for Ob or leptin in feline. Please contact our Technical Service Department if you can provide a protein sequence and we can perform sequence analysis with the antigen to predict any potential cross-reactivity with this species. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-11-03

Can Ob (F-3): sc-48408 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Ob (F-3): sc-48408 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from buon prodottoho ricevuto una aliquota di prova e l'ho impiegata su tessuti di coniglio con ottimi risultati ad una concentrazione di 1:800
Date published: 2022-06-27
Rated 4 out of 5 by from Ottimo in diluizione 1:50Ho utilizzato l'anticorpo su oociti di diversa specie animale Allego foto di microscopia confocale 40x Protocollo utilizzato: Fissazione in PFA al 4% Pretrattamento in blocking per 4-5 ore (triton-X 0,1%, BSA 0,5%, Glicina 2%, Sodium Azide 0,2 % e Siero 2%) Incubazione primario over night a 4 °C 3 Lavaggi per 30' Incubazione secondario 1:100 antimouse coniugato con alexafluor 488 - 2 ore a 37° 3 lavaggi per 30' . Montaggio in vetrino
Date published: 2021-08-04
Rated 1 out of 5 by from can not detect specific band by WBit can not detect a specific band by WB in our lab.
Date published: 2017-09-18
Rated 1 out of 5 by from IHC Assay Review for Ob mABApplication: Immunohistochemistry. Protocol: Standard protocol for saliva smear staining. Dilutions tested: 1:10, 1:30, 1:90, 1:270. Results: No staining observed.
Date published: 2017-08-31
Rated 5 out of 5 by from Amazing IHC staining!Clean and specific staining on paraffin tissue sections. Really strong and reliable. Great choice!
Date published: 2017-01-25
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human ovary tissue. -SCBT QC
Date published: 2015-02-01
Rated 5 out of 5 by from Great Western Blot data of human recombinant ObGreat Western Blot data of human recombinant Ob. -SCBT QC
Date published: 2014-10-09
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Ob 抗体 (F-3) is rated 3.7 out of 5 by 7.
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