Date published: 2025-11-1

1-800-457-3801

SCBT Portrait Logo
Seach Input

o-Phenylenediamine (CAS 95-54-5)

0.0(0)
Write a reviewAsk a question

Alternate Names:
1,2-Phenylenediamine; OPD
Application:
o-Phenylenediamine is used to detect horseradish peroxidase activity
CAS Number:
95-54-5
Molecular Weight:
108.14
Molecular Formula:
C6H8N2
Supplemental Information:
This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

QUICK LINKS

o-Phenylenediamine functions as a primary intermediate in the production of various dyes, pigments, and polymers. It acts as a precursor for the synthesis of hair dyes, textile dyes, and rubber antioxidants. o-Phenylenediamine is utilized for its ability to undergo oxidative coupling reactions to form heterocyclic compounds. O-Phenylenediamine is a building block in the formation of polybenzimidazoles, which are high-performance polymers with exceptional thermal and chemical stability. At the molecular level, o-Phenylenediamine participates in electrophilic aromatic substitution reactions, enabling the formation of complex organic molecules with diverse applications in materials science and industrial chemistry.


o-Phenylenediamine (CAS 95-54-5) References

  1. Treatment of wastewater containing o-phenylenediamine by ozone in a rotor-stator reactor.  |  Arowo, M., et al. 2016. Water Sci Technol. 73: 1357-63. PMID: 27003076
  2. Tuning the optical properties of poly(o-phenylenediamine-co-pyrrole) via template mediated copolymerization.  |  Jadoun, S., et al. 2018. Des Monomers Polym. 21: 75-81. PMID: 29760604
  3. Robust magnetic laccase-mimicking nanozyme for oxidizing o-phenylenediamine and removing phenolic pollutants.  |  Zhang, S., et al. 2020. J Environ Sci (China). 88: 103-111. PMID: 31862051
  4. o-Phenylenediamine/gold nanocluster-based nanoplatform for ratiometric fluorescence detection of alkaline phosphatase activity.  |  Han, X., et al. 2020. Talanta. 212: 120768. PMID: 32113538
  5. Fluorescent and Colorimetric Sensors Based on the Oxidation of o-Phenylenediamine.  |  Ye, Q., et al. 2020. ACS Omega. 5: 20698-20706. PMID: 32875203
  6. Catalytic oxidation of O-phenylenediamine by silver nanoparticles for resonance Rayleigh scattering detection of mercury (II) in water samples.  |  Al-Onazi, WA. and Abdel-Lateef, MA. 2022. Spectrochim Acta A Mol Biomol Spectrosc. 264: 120258. PMID: 34384994
  7. Unveiling the Actual Catalytic Sites in Nanozyme-Catalyzed Oxidation of o-Phenylenediamine.  |  Cheng, L., et al. 2021. Small. 17: e2104083. PMID: 34655154
  8. The Oxidation Mechanism of Flavan-3-ols by an Enzymatic Reaction Using Liquid Chromatography-Mass Spectrometry-Based Metabolomics Combined with Captured o-Quinone Intermediates of Flavan-3-ols by o-Phenylenediamine.  |  Zha, M., et al. 2022. J Agric Food Chem. 70: 5715-5727. PMID: 35475606
  9. Utilization of the peroxidase-like activity of silver nanoparticles nanozyme on O-phenylenediamine/H2O2 system for fluorescence detection of mercury (II) ions.  |  Abdel-Lateef, MA. 2022. Sci Rep. 12: 6953. PMID: 35484380
  10. Fluorescent Immunoassay with a Copper Polymer as the Signal Label for Catalytic Oxidation of O-Phenylenediamine.  |  Feng, Y., et al. 2022. Molecules. 27: PMID: 35744801
  11. In Situ Formation of o-Phenylenediamine Cascade Polymers Mediated by Metal-Organic Framework Nanozymes for Fluorescent and Photothermal Dual-Mode Assay of Acetylcholinesterase Activity.  |  Li, S., et al. 2022. Anal Chem. 94: 17263-17271. PMID: 36463539
  12. Synthetic aspects of 1,4- and 1,5-benzodiazepines using o-phenylenediamine: a study of past quinquennial.  |  Teli, S., et al. 2023. RSC Adv. 13: 3694-3714. PMID: 36756601
  13. Molecularly imprinted sensor based on poly-o-phenylenediamine-hydroquinone polymer for β-amyloid-42 detection.  |  Ding, M., et al. 2023. Anal Bioanal Chem. 415: 1545-1557. PMID: 36808273
  14. 2,3-Diaminophenazine is the product from the horseradish peroxidase-catalyzed oxidation of o-phenylenediamine.  |  Tarcha, PJ., et al. 1987. Anal Biochem. 165: 230-3. PMID: 3688435
  15. Optimizing the o-phenylenediamine assay for horseradish peroxidase: effects of phosphate and pH, substrate and enzyme concentrations, and stopping reagents.  |  Bovaird, JH., et al. 1982. Clin Chem. 28: 2423-6. PMID: 6754137
  16. Determination of urinary oxalate by high-performance liquid chromatography.  |  Hughes, H., et al. 1982. Anal Biochem. 119: 1-3. PMID: 7072931
  17. Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen.  |  Wolters, G., et al. 1976. J Clin Pathol. 29: 873-9. PMID: 789402
  18. Cysteine conjugate toxicity in a human cell line: correlation with C-S lyase activity in human hepatic tissue.  |  Buckberry, LD., et al. 1993. Hum Exp Toxicol. 12: 329-35. PMID: 8104012
  19. o-Phenylenediamine-modified carbon fiber electrodes for the detection of nitric oxide.  |  Friedemann, MN., et al. 1996. Anal Chem. 68: 2621-8. PMID: 8694261

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

o-Phenylenediamine, 50 g

sc-215611
50 g
$139.00

o-Phenylenediamine, 100 g

sc-215611A
100 g
$255.00