O-Nitrophenyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside (CAS 3053-17-6)

O-Nitrophenyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside is a synthetic compound used extensively in enzymology research, particularly in studies involving β-galactosidase enzymes and carbohydrate metabolism. This chemical serves as a substrate for β-galactosidases, which catalyze the hydrolysis of β-galactosyl linkages in various glycoconjugates. Through enzymatic assays, researchers can investigate the substrate specificity and catalytic mechanisms of β-galactosidases, shedding light on their roles in lactose metabolism, glycoprotein processing, and cellular signaling pathways. Additionally, O-Nitrophenyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside has been instrumental in the development of high-throughput screening assays for the discovery and characterization of β-galactosidase inhibitors, with potential applications in agriculture, biotechnology, and the food industry. Furthermore, its strategic use in structural biology studies and molecular modeling facilitates the elucidation of enzyme-substrate interactions and the design of novel enzyme inhibitors. Overall, this compound plays a crucial role in advancing our understanding of β-galactosidase-mediated carbohydrate metabolism and in the development of research methodologies for diverse enzymology studies in the field of glycobiology.