O-(2-Aminoethyl)-O′-(2-azidoethyl)pentaethylene glycol (CAS 957486-82-7)

O-(2-Aminoethyl)-O′-(2-azidoethyl)pentaethylene glycol is a polyethylene glycol (PEG) derivative featuring both aminoethyl and azidoethyl functional groups. This compound provides versatile reactive handles that are particularly advantageous in bioconjugation and click chemistry applications. The aminoethyl group serves as a primary amine, readily reacting with carboxyl-containing biomolecules to form stable amide bonds. Simultaneously, the azidoethyl group participates in copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reactions, commonly known as click chemistry, enabling highly specific conjugation with alkyne-containing molecules. In research, this compound is valuable for studying molecular interactions and protein modification. The pentaethylene glycol backbone offers water solubility and steric flexibility, which is critical for maintaining the native activity of conjugated biomolecules. This non-fouling property makes it particularly useful for modifying surfaces in biosensors and diagnostic assays. Researchers use O-(2-Aminoethyl)-O′-(2-azidoethyl)pentaethylene glycol to conjugate fluorescent dyes, antibodies, or peptides onto surfaces, enhancing assay specificity and enabling the detection of biological targets with high sensitivity. Additionally, this compound′s click-reactive azide functionality allows for rapid and efficient attachment of various ligands or probes, facilitating the exploration of diverse biochemical pathways and interactions. It has been instrumental in developing biocompatible interfaces and exploring the effect of controlled pegylation on protein and surface interactions in proteomics and molecular biology research.