Nup54 Antibody (613J2U): sc-517605

Nup54 Antibody (613J2U) is a mouse monoclonal IgG1 antibody that detects Nup54 in human samples through western blotting (WB). Nup54, or nucleoporin 54kDa, is a crucial component of the nuclear pore complex (NPC), which facilitates the selective transport of macromolecules between the nucleus and cytoplasm in eukaryotic cells. This 507 amino acid protein is primarily localized in the nucleus, where Nup54 plays a vital role in maintaining cellular homeostasis by regulating the passage of proteins and RNA across the nuclear membrane. The importance of Nup54 extends beyond its structural role; Nup54 exists in multiple alternatively spliced isoforms, which allows for functional diversity and adaptability in cellular processes. Additionally, Nup54 undergoes post-translational O-glycosylation, a modification that can influence interactions with other proteins and overall function within the NPC. Understanding the dynamics of Nup54 and its isoforms is essential for elucidating the mechanisms of nucleocytoplasmic transport and implications in various cellular functions and diseases. Anti-Nup54 antibody (613J2U) is an invaluable tool for researchers investigating these critical processes.

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Nup54 Antibody (613J2U) References:

1. The stoichiometry of the nucleoporin 62 subcomplex of the nuclear pore in solution.  |  Ulrich, A., et al. 2014. Mol Biol Cell. 25: 1484-92. PMID: 24574455
2. Ordered Regions of Channel Nucleoporins Nup62, Nup54, and Nup58 Form Dynamic Complexes in Solution.  |  Sharma, A., et al. 2015. J Biol Chem. 290: 18370-8. PMID: 26025361
3. Crystal structure of the metazoan Nup62•Nup58•Nup54 nucleoporin complex.  |  Chug, H., et al. 2015. Science. 350: 106-10. PMID: 26292704
4. The Nup62 Coiled-Coil Motif Provides Plasticity for Triple-Helix Bundle Formation.  |  Dewangan, PS., et al. 2017. Biochemistry. 56: 2803-2811. PMID: 28406021
5. Nucleoporin 54 contributes to homologous recombination repair and post-replicative DNA integrity.  |  Rodriguez-Berriguete, G., et al. 2018. Nucleic Acids Res. 46: 7731-7746. PMID: 29986057
6. Conformation of the nuclear pore in living cells is modulated by transport state.  |  Pulupa, J., et al. 2020. Elife. 9: PMID: 33346731
7. Nup54-induced CARM1 nuclear importation promotes gastric cancer cell proliferation and tumorigenesis through transcriptional activation and methylation of Notch2.  |  Wang, F., et al. 2022. Oncogene. 41: 246-259. PMID: 34725461
8. Indel driven rapid evolution of core nuclear pore protein gene promoters.  |  McQuarrie, DWJ., et al. 2023. Sci Rep. 13: 8035. PMID: 37198214
9. CircNUP54 promotes hepatocellular carcinoma progression via facilitating HuR cytoplasmic export and stabilizing BIRC3 mRNA.  |  Tang, C., et al. 2024. Cell Death Dis. 15: 191. PMID: 38443362
10. The role of ABI2 in modulating nuclear proteins: Therapeutic implications for NUP54 and NUP153 in TNBC.  |  Elavarasu, SM., et al. 2025. Adv Protein Chem Struct Biol. 143: 97-115. PMID: 39843146