NUCKS Double Nickase Plasmid (h): sc-413018-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NUCKS Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NUCKS Double Nickase Plasmid (h) and NUCKS Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NUCKS1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NUCKS Double Nickase Plasmid (h) | sc-413018-NIC | 20 µg | $410.00 | |||
NUCKS Double Nickase Plasmid (h2) | sc-413018-NIC-2 | 20 µg | $410.00 |
NUCKS1 encodes NUCKS, a nuclear chromatin-associated protein implicated in the coordination of transcriptional regulation with DNA replication and repair. NUCKS is reported to be phosphorylated in a cell cycle-dependent manner and to participate in chromatin remodeling and genome maintenance processes that interface with DNA damage response signaling. Altered NUCKS1 expression has been linked to proliferative phenotypes and has been observed across multiple cancer contexts, supporting its relevance for studying cell cycle control, stress responses, and tumor-associated regulatory networks. As a nuclear factor with broad regulatory reach, NUCKS1 is frequently investigated for its contributions to transcriptional programs that shape growth, survival, and genomic stability.
NUCKS Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NUCKS1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NUCKS1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NUCKS1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NUCKS1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.