NTR2 CRISPR/Cas9 KO Plasmid (h): sc-405783
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NTR2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NTR2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NTR2 CRISPR/Cas9 KO Plasmid (h) | sc-405783 | 20 µg | $397.00 |
Overview
Neurotensin receptor 2 (NTSR2, NTR2) is a class A G protein-coupled receptor that binds the neuropeptide neurotensin and couples predominantly to Gαq/11 to stimulate phospholipase C signaling, intracellular calcium mobilization, and downstream kinase cascades such as PKC–MAPK/ERK. NTR2 signaling influences neuronal excitability, synaptic transmission, and neuroendocrine responses, and it can modulate crosstalk with dopamine and glutamate pathways that shape circuit-level activity. In non-neuronal contexts, NTSR2 expression has been linked to inflammatory signaling and cellular stress responses, supporting its relevance to studies of neuroinflammation and tissue remodeling. Dysregulated neurotensin–NTR2 axis activity has been investigated in neurological and neuropsychiatric disease biology and in mechanisms that contribute to altered cell proliferation and survival in disease-associated models.
NTR2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the NTSR2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the NTSR2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the NTSR2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NTR2 protein expression.
This CRISPR knockout system enables efficient generation of NTSR2-deficient cell models for investigation of NTR2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting NTSR2 exon(s) critical for NTR2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple NTSR2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NTR2 CRISPR/Cas9 KO Plasmid (h) and NTR2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the NTSR2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NTR2 HDR Plasmid (h) and NTR2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by NTSR2 homology arms to support homology-directed repair at defined NTSR2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.