NTH1 Antibody (2660C1a): sc-130644

NTH1 Antibody (2660C1a) is a mouse monoclonal IgG1 antibody that detects NTH1 protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). NTH1 (2660C1a) antibody is available as a non-conjugated anti-NTH1 antibody. The human endonuclease III (hNTH1) is a crucial DNA glycosylase that plays a significant role in maintaining genomic integrity by recognizing and excising oxidatively damaged pyrimidines from DNA. hNTH1 is essential for the repair of DNA lesions that can lead to mutations and contribute to various diseases, including cancer. hNTH1 operates through a beta-elimination mechanism, forming a Schiff base with the deoxyribose at the active site, specifically at Lysine-212, after base removal. Notably, full-length human NTH1 is predominantly localized in the nucleus, which is vital for DNA repair, while the mouse variant is primarily found in the mitochondria, with a smaller fraction in the nucleus. This localization difference is attributed to the presence of a nuclear localization sequence in human NTH1 that is absent in the mouse version. Furthermore, NTH1 gene is situated adjacent to the TSC2 gene, which is implicated in tuberous sclerosis, and both genes share a bidirectional promoter essential for transcription. The ability of NTH1 to excise damaged bases underscores its importance in cellular defense against genomic instability, making anti-NTH1 antibody (2660C1a) a valuable tool for researchers studying DNA repair mechanisms and related pathologies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

NTH1 Antibody (2660C1a) References:

1. Identification of functional elements in the bidirectional promoter of the mouse Nthl1 and Tsc2 genes.  |  Ikeda, S., et al. 2000. Biochem Biophys Res Commun. 273: 1063-8. PMID: 10891372
2. Differential intracellular localization of the human and mouse endonuclease III homologs and analysis of the sorting signals.  |  Ikeda, S., et al. 2002. DNA Repair (Amst). 1: 847-54. PMID: 12531031
3. Human NTH1 physically interacts with p53 and proliferating cell nuclear antigen.  |  Oyama, M., et al. 2004. Biochem Biophys Res Commun. 321: 183-91. PMID: 15358233
4. DNA repair mechanisms in dividing and non-dividing cells.  |  Iyama, T. and Wilson, DM. 2013. DNA Repair (Amst). 12: 620-36. PMID: 23684800
5. NTH1 Is a New Target for Ubiquitylation-Dependent Regulation by TRIM26 Required for the Cellular Response to Oxidative Stress.  |  Williams, SC. and Parsons, JL. 2018. Mol Cell Biol. 38: PMID: 29610152
6. An Excimer Clamp for Measuring Damaged-Base Excision by the DNA Repair Enzyme NTH1.  |  Jun, YW., et al. 2020. Angew Chem Int Ed Engl. 59: 7450-7455. PMID: 32109332
7. Human endonuclease III/NTH1: focusing on the [4Fe-4S] cluster and the N-terminal domain.  |  Moe, E., et al. 2022. Chem Commun (Camb). 58: 12568-12571. PMID: 36279116
8. Purification and characterization of human NTH1, a homolog of Escherichia coli endonuclease III. Direct identification of Lys-212 as the active nucleophilic residue.  |  Ikeda, S., et al. 1998. J Biol Chem. 273: 21585-93. PMID: 9705289
9. Cloning and characterization of a mouse homologue (mNthl1) of Escherichia coli endonuclease III.  |  Sarker, AH., et al. 1998. J Mol Biol. 282: 761-74. PMID: 9743625
10. The discovery of a new family of mammalian enzymes for repair of oxidatively damaged DNA, and its physiological implications.  |  Hazra, TK., et al. 2003. Carcinogenesis. 24: 155-7. PMID: 12584162

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NTH1 Antibody (2660C1a) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-NTH1 Antibody (2660C1a) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NTH1 Antibody (2660C1a) Specifications

Alternate Product Name: NTH1

Product Clone Name: 2660C1a

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: NTH1

Epitope Species: human

Product Reactivity/Detection: NTH1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 1.0% stabilizer protein

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for NTH1 Antibody (2660C1a): N/A