NT5C2 Antibody (FF-12): sc-82000

NT5C2 Antibody (FF-12) is a mouse monoclonal IgG2a kappa light chain antibody that detects NT5C2 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-NT5C2 antibody (FF-12) is available as the non-conjugated form. NT5C2, also known as 5′-nucleotidase, cytosolic II, NT5B, NT5CP, or PNT5, is a crucial enzyme composed of 561 amino acids that plays a vital role in nucleotide metabolism by catalyzing the hydrolysis of nucleotides into nucleosides and phosphates. This enzymatic activity is essential for maintaining the balance of purine and pyrimidine nucleotides within cells, which is critical for various cellular processes, including DNA and RNA synthesis, energy metabolism, and signal transduction. NT5C2 is allosterically activated by ATP and other compounds, allowing for fine-tuned regulation of inosine monophosphate (IMP) and guanosine monophosphate (GMP) levels in response to cellular needs. Structurally, NT5C2 shares similarities with enzymes from the haloacid dehalogenase superfamily, which are known for their hydrolase activity. NT5C2 localizes in the cytoplasmic matrix, ensuring accessibility to substrates and supporting cellular metabolism. Mutations in NT5C2 can lead to elevated uric acid levels, potentially resulting in conditions such as gout and hyperuricemia, highlighting NT5C2′s significance in human health.

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NT5C2 Antibody (FF-12) References:

1. cN-II expression predicts survival in patients receiving gemcitabine for advanced non-small cell lung cancer.  |  Sève, P., et al. 2005. Lung Cancer. 49: 363-70. PMID: 15923058
2. The prognostic value of cN-II and cN-III enzymes in adult acute myeloid leukemia.  |  Galmarini, CM., et al. 2005. Haematologica. 90: 1699-701. PMID: 16330448
3. F-ara-AMP is a substrate of cytoplasmic 5'-nucleotidase II (cN-II): HPLC and NMR studies of enzymatic dephosphorylation.  |  Jordheim, LP., et al. 2006. Nucleosides Nucleotides Nucleic Acids. 25: 289-97. PMID: 16629121
4. Crystal structure of human cytosolic 5'-nucleotidase II: insights into allosteric regulation and substrate recognition.  |  Walldén, K., et al. 2007. J Biol Chem. 282: 17828-36. PMID: 17405878
5. What does over-expression of cN-II enzyme signify in haematological malignancies?  |  Galmarini, CM. 2007. Leuk Res. 31: 1325-6. PMID: 17434583
6. Molecular cloning of human cytosolic purine 5'-nucleotidase.  |  Oka, J., et al. 1994. Biochem Biophys Res Commun. 205: 917-22. PMID: 7999131

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NT5C2 Antibody (FF-12) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-NT5C2 Antibody (FF-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NT5C2 Antibody (FF-12) Specifications

Alternate Product Name: NT5C2

Product Clone Name: FF-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-562

Epitope Target: NT5C2

Epitope Species: human

Product Reactivity/Detection: NT5C2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 50 µg IgG_2a kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for NT5C2 Antibody (FF-12): N/A