NSP3 CRISPR/Cas9 KO Plasmid (h): sc-407380
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NSP3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NSP3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NSP3 Antibody (J-16.1): sc-100792
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NSP3 CRISPR/Cas9 KO Plasmid (h) | sc-407380 | 20 µg | $397.00 |
Overview
SH2D3C encodes NSP3, a cytosolic adaptor protein containing SH2 and SH3 domains that helps couple tyrosine-phosphorylated receptors to downstream signaling complexes. NSP3 participates in pathways governing cytoskeletal remodeling, integrin-dependent adhesion, and receptor-mediated signal transduction, influencing cell migration and immune-related cellular behaviors. Through interactions with kinases and scaffold proteins, it can modulate MAPK and other phosphotyrosine-driven networks that shape activation thresholds and cellular motility programs. Dysregulated adaptor signaling and altered adhesion/migration phenotypes are frequently implicated in inflammatory biology and cancer-associated processes, making SH2D3C a useful target for mechanistic studies of signaling circuitry.
NSP3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SH2D3C gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SH2D3C together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SH2D3C open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NSP3 protein expression.
This CRISPR knockout system enables efficient generation of SH2D3C-deficient cell models for investigation of NSP3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SH2D3C exon(s) critical for NSP3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SH2D3C genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NSP3 CRISPR/Cas9 KO Plasmid (h) and NSP3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SH2D3C locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NSP3 HDR Plasmid (h) and NSP3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SH2D3C homology arms to support homology-directed repair at defined SH2D3C target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.