NSF Antibody (C-5): sc-74457

NSF Antibody (C-5) is a mouse monoclonal IgG2a light chain antibody that detects NSF in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NSF, or N-ethylmaleimide-sensitive factor, plays a crucial role in the process of membrane fusion, which is essential for various cellular functions, including neurotransmitter release and vesicle trafficking. NSF is primarily located in the cytoplasm, where NSF interacts with soluble NSF attachment proteins (SNAPs) and syntaxins to facilitate the assembly of the SNARE complex, a key component in the exocytosis pathway. Proper functioning of NSF is vital for maintaining synaptic transmission and cellular communication, making NSF a significant target for research in neurobiology and cell biology. Anti-NSF antibody (C-5) provides a reliable tool for studying the dynamics of membrane fusion and the molecular mechanisms underlying synaptic function, thereby contributing to our understanding of various physiological and pathological processes.

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NSF Antibody (C-5) References:

1. N-ethylmaleimide sensitive factor (NSF) structure and function.  |  Whiteheart, SW., et al. 2001. Int Rev Cytol. 207: 71-112. PMID: 11352269
2. Uncoupling the ATPase activity of the N-ethylmaleimide sensitive factor (NSF) from 20S complex disassembly.  |  Matveeva, EA., et al. 2002. Biochemistry. 41: 530-6. PMID: 11781091
3. Requirements for the catalytic cycle of the N-ethylmaleimide-Sensitive Factor (NSF).  |  Zhao, C., et al. 2012. Biochim Biophys Acta. 1823: 159-71. PMID: 21689688
4. Potential cellular functions of N-ethylmaleimide sensitive factor in the photoreceptor.  |  Huang, SP. and Craft, CM. 2012. Adv Exp Med Biol. 723: 791-7. PMID: 22183408
5. The Neurexin/N-Ethylmaleimide-sensitive Factor (NSF) Interaction Regulates Short Term Synaptic Depression.  |  Li, T., et al. 2015. J Biol Chem. 290: 17656-17667. PMID: 25953899
6. The N-ethylmaleimide-sensitive factor and dysbindin interact to modulate synaptic plasticity.  |  Gokhale, A., et al. 2015. J Neurosci. 35: 7643-53. PMID: 25972187
7. Recent Advances in Deciphering the Structure and Molecular Mechanism of the AAA+ ATPase N-Ethylmaleimide-Sensitive Factor (NSF).  |  Zhao, M. and Brunger, AT. 2016. J Mol Biol. 428: 1912-26. PMID: 26546278
8. N-Ethylmaleimide Sensitive Factor (NSF) Inhibition Prevents Vascular Instability following Gram-Positive Pulmonary Challenge.  |  Lee, JY., et al. 2016. PLoS One. 11: e0157837. PMID: 27355324
9. A connection between reversible tyrosine phosphorylation and SNARE complex disassembly activity of N-ethylmaleimide-sensitive factor unveiled by the phosphomimetic mutant N-ethylmaleimide-sensitive factor-Y83E.  |  Ruete, MC., et al. 2019. Mol Hum Reprod. 25: 344-358. PMID: 31194868
10. N-Ethylmaleimide-sensitive factor (NSF) and alpha-soluble NSF attachment proteins (SNAP) mediate dissociation of GS28-syntaxin 5 Golgi SNAP receptors (SNARE) complex.  |  Subramaniam, VN., et al. 1997. J Biol Chem. 272: 25441-4. PMID: 9325254

The preferred NSF antibody is NSF Antibody (A-3): sc-515043.