NRSF Antibody (F-3): sc-374611

NRSF Antibody (F-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects NRSF protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-NRSF antibody (F-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NRSF protein, also known as neuron-restrictive silencer factor or RE1-silencing transcription factor, plays a crucial role in regulating gene expression by binding to the neuron-restrictive silencer element (NRSE) in non-neuronal cells, thereby repressing neuronal gene transcription. This function is vital for maintaining the identity of non-neuronal cells and preventing premature neuronal differentiation. NRSF is predominantly expressed in non-neural tissues but is also found in developing neurons and at lower levels in the brain, highlighting its importance in both neural and non-neural contexts. Structurally, NRSF contains nine zinc-finger domains, which are essential for DNA-binding capability, and can exist in a truncated form known as REST4, produced through alternative splicing. While REST4 retains some functionality, REST4 binds DNA weakly and is still transported to the nucleus, indicating a complex regulatory mechanism. The interaction of NRSF with corepressors such as mSin3 and histone deacetylases (HDAC) suggests involvement in broader regulatory networks beyond the nervous system. Down-regulation of NRSF during neural differentiation is critical for proper development of specific neuronal classes, underscoring NRSF′s role as a key regulator in both embryonic development and neurogenesis.

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NRSF Antibody (F-3) References:

1. Transcriptional repression by REST: recruitment of Sin3A and histone deacetylase to neuronal genes.  |  Huang, Y., et al. 1999. Nat Neurosci. 2: 867-72. PMID: 10491605
2. Studies on the interaction of REST4 with the cholinergic repressor element-1/neuron restrictive silencer element.  |  Lee, JH., et al. 2000. Brain Res Mol Brain Res. 80: 88-98. PMID: 11039732
3. Constitutive expression of the neuron-restrictive silencer factor (NRSF)/REST in differentiating neurons disrupts neuronal gene expression and causes axon pathfinding errors in vivo.  |  Paquette, AJ., et al. 2000. Proc Natl Acad Sci U S A. 97: 12318-23. PMID: 11050251
4. Role of zinc finger domains of the transcription factor neuron-restrictive silencer factor/repressor element-1 silencing transcription factor in DNA binding and nuclear localization.  |  Shimojo, M., et al. 2001. J Biol Chem. 276: 13121-6. PMID: 11145971
5. The neuron-restrictive silencer element-neuron-restrictive silencer factor system regulates basal and endothelin 1-inducible atrial natriuretic peptide gene expression in ventricular myocytes.  |  Kuwahara, K., et al. 2001. Mol Cell Biol. 21: 2085-97. PMID: 11238943
6. Repressor element silencing transcription factor/neuron-restrictive silencing factor (REST/NRSF) can act as an enhancer as well as a repressor of corticotropin-releasing hormone gene transcription.  |  Seth, KA. and Majzoub, JA. 2001. J Biol Chem. 276: 13917-23. PMID: 11278361
7. Brain REST/NRSF Is Not Only a Silent Repressor but Also an Active Protector.  |  Zhao, Y., et al. 2017. Mol Neurobiol. 54: 541-550. PMID: 26742529
8. NRSF and Its Epigenetic Effectors: New Treatments for Neurological Disease.  |  Thompson, R. and Chan, C. 2018. Brain Sci. 8: PMID: 30572571
9. REST/NRSF drives homeostatic plasticity of inhibitory synapses in a target-dependent fashion.  |  Prestigio, C., et al. 2021. Elife. 10: PMID: 34855580
10. Transcription factor network analysis identifies REST/NRSF as an intrinsic regulator of CNS regeneration in mice.  |  Cheng, Y., et al. 2022. Nat Commun. 13: 4418. PMID: 35906210
11. The neuron-restrictive silencer factor (NRSF): a coordinate repressor of multiple neuron-specific genes.  |  Schoenherr, CJ. and Anderson, DJ. 1995. Science. 267: 1360-3. PMID: 7871435
12. NRSF/REST is required in vivo for repression of multiple neuronal target genes during embryogenesis.  |  Chen, ZF., et al. 1998. Nat Genet. 20: 136-42. PMID: 9771705

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NRSF Antibody (F-3) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-NRSF Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NRSF Antibody (F-3) Specifications

Alternate Product Name: RE1-Silencing Transcription factor (REST)

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-290

Epitope Target: NRSF

Epitope Species: human

Product Reactivity/Detection: NRSF

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for NRSF Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent