Nrf3 Double Nickase Plasmid (h): sc-404543-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nrf3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nrf3 Double Nickase Plasmid (h) and Nrf3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NFE2L3. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nrf3 Double Nickase Plasmid (h) | sc-404543-NIC | 20 µg | $410.00 | |||
Nrf3 Double Nickase Plasmid (h2) | sc-404543-NIC-2 | 20 µg | $410.00 |
NFE2L3 encodes the cap’n’collar bZIP transcription factor Nrf3, a regulator of redox-responsive gene expression and proteostasis programs. Nrf3 participates in oxidative stress signaling and interfaces with ER-associated processes, including transcriptional control of proteasome subunits that shape protein turnover and cellular adaptation. In human cells, dysregulated Nrf3 activity has been linked to altered proliferation, differentiation, and stress tolerance, making it relevant to studies of tumor biology and inflammatory microenvironments. Its pathway connectivity to antioxidant response networks and ubiquitin–proteasome function supports mechanistic investigation of how cells maintain homeostasis under metabolic and genotoxic stress.
Nrf3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NFE2L3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NFE2L3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NFE2L3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NFE2L3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.