Nrf2 Double Nickase Plasmid (m): sc-421869-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nrf2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nrf2 Double Nickase Plasmid (m) and Nrf2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Nfe2l2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nrf2 Double Nickase Plasmid (m) | sc-421869-NIC | 20 µg | $410.00 | |||
Nrf2 Double Nickase Plasmid (m2) | sc-421869-NIC-2 | 20 µg | $410.00 |
Mouse Nfe2l2 encodes Nrf2, a bZIP transcription factor that coordinates cellular defense against electrophilic and oxidative stress by binding antioxidant response elements (AREs) and inducing detoxification, redox, and proteostasis genes. Under basal conditions, Nrf2 activity is restrained through KEAP1-dependent ubiquitination and proteasomal turnover, while stress signals stabilize Nrf2 to remodel transcriptional programs across glutathione metabolism, NADPH regeneration, and xenobiotic clearance pathways. Nrf2 signaling intersects with inflammatory and metabolic networks, including cross-talk with NF-κB and mitochondrial homeostasis, shaping adaptive responses to environmental and endogenous stressors. Dysregulation of the KEAP1–Nrf2 axis is implicated in models of carcinogenesis, chronic inflammatory states, neurodegeneration, and metabolic dysfunction, making Nfe2l2 a common target for mechanistic studies of stress resilience and redox biology.
Nrf2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Nfe2l2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Nfe2l2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Nfe2l2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Nfe2l2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.