NRF-1 Antibody (147.1): sc-101102

NRF-1 Antibody (147.1) is a mouse monoclonal IgG1 kappa light chain antibody that detects NRF-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). NRF-1 (147.1) antibody is available as the non-conjugated form. Nuclear respiratory factor-1 (NRF-1) serves as a crucial transcriptional activator, playing a significant role in the regulation of nuclear genes essential for mitochondrial respiratory function and overall cellular metabolism. NRF-1 is primarily located in the nucleus, where NRF-1 orchestrates the expression of genes involved in the respiratory chain, thereby influencing energy production within cells. Proper functioning of NRF-1 is vital, particularly during early embryogenesis, as NRF-1 is necessary for maintaining mitochondrial DNA integrity and respiratory chain activity. Studies have shown that while wild-type and NRF-1+/- blastocysts can develop normally in vitro, NRF-1-/- blastocysts fail to do so, highlighting NRF-1′s essential role in early developmental processes. NRF-1 is responsive to physiological signals, enabling cells to adapt their energy-generating capacity according to metabolic demands, making NRF-1 a key player in cellular energy homeostasis. NRF-1 is recognized as a major transcription factor that binds to specific promoters in tissues such as the brain and testis, underscoring NRF-1′s importance in various biological contexts.

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NRF-1 Antibody (147.1) References:

1. Respiratory uncoupling induces delta-aminolevulinate synthase expression through a nuclear respiratory factor-1-dependent mechanism in HeLa cells.  |  Li, B., et al. 1999. J Biol Chem. 274: 17534-40. PMID: 10364186
2. Multiple 5'-untranslated exons in the nuclear respiratory factor 1 gene span 47 kb and contribute to transcript heterogeneity and translational efficiency.  |  Huo, L. and Scarpulla, RC. 1999. Gene. 233: 213-24. PMID: 10375638
3. Dynein light chain interacts with NRF-1 and EWG, structurally and functionally related transcription factors from humans and drosophila.  |  Herzig, RP., et al. 2000. J Cell Sci. 113 Pt 23: 4263-73. PMID: 11069771
4. Mitochondrial DNA instability and peri-implantation lethality associated with targeted disruption of nuclear respiratory factor 1 in mice.  |  Huo, L. and Scarpulla, RC. 2001. Mol Cell Biol. 21: 644-54. PMID: 11134350
5. NRF1 Is an ER Membrane Sensor that Is Central to Cholesterol Homeostasis.  |  Widenmaier, SB., et al. 2017. Cell. 171: 1094-1109.e15. PMID: 29149604
6. ATF4 activation promotes hepatic mitochondrial dysfunction by repressing NRF1-TFAM signalling in alcoholic steatohepatitis.  |  Hao, L., et al. 2021. Gut. 70: 1933-1945. PMID: 33177163
7. Mitophagy receptor FUNDC1 is regulated by PGC-1α/NRF1 to fine tune mitochondrial homeostasis.  |  Liu, L., et al. 2021. EMBO Rep. 22: e50629. PMID: 33554448
8. Nrf1 promotes heart regeneration and repair by regulating proteostasis and redox balance.  |  Cui, M., et al. 2021. Nat Commun. 12: 5270. PMID: 34489413
9. NRF1-mediated microglial activation triggers high-altitude cerebral edema.  |  Wang, X., et al. 2022. J Mol Cell Biol. 14: PMID: 35704676
10. NRF1-mediated mitochondrial biogenesis antagonizes innate antiviral immunity.  |  Zhao, T., et al. 2023. EMBO J. 42: e113258. PMID: 37409632
11. NFE2L1/Nrf1 serves as a potential therapeutical target for neurodegenerative diseases.  |  Łuczyńska, K., et al. 2024. Redox Biol. 69: 103003. PMID: 38150994
12. Sugar-mediated non-canonical ubiquitination impairs Nrf1/NFE2L1 activation.  |  Yoshida, Y., et al. 2024. Mol Cell. 84: 3115-3127.e11. PMID: 39116872

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-NRF-1 Antibody (147.1) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-NRF-1 Antibody (147.1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

NRF-1 Antibody (147.1) Specifications

Alternate Product Name: Nuclear Respiratory Factor 1

Product Clone Name: 147.1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 201-286

Epitope Target: NRF-1

Epitope Species: human

Product Reactivity/Detection: NRF-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 50 µg IgG₁ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for NRF-1 Antibody (147.1): N/A