Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NRAMP 2 Double Nickase Plasmid (h): sc-418176-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NRAMP 2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NRAMP 2 Double Nickase Plasmid (h) and NRAMP 2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SLC11A2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NRAMP 2 Antibody (G-5): sc-166884
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NRAMP 2 Double Nickase Plasmid (h) | sc-418176-NIC | 20 µg | $410.00 | |||
NRAMP 2 Double Nickase Plasmid (h2) | sc-418176-NIC-2 | 20 µg | $410.00 |
SLC11A2 encodes NRAMP2 (DMT1), a proton-coupled divalent metal transporter that mediates cellular uptake of ferrous iron and other transition metals across endosomal and plasma membranes. NRAMP2 supports transferrin-dependent iron release from endosomes and contributes to systemic iron handling in concert with ferrireductases and iron export machinery, linking it to iron homeostasis, oxidative stress balance, and metabolic regulation. Altered SLC11A2 activity is associated with disorders of iron metabolism, including microcytic anemia and tissue iron overload phenotypes, and has been studied in contexts such as neurodegeneration and inflammation where metal trafficking can modulate cellular vulnerability. In human cells, NRAMP2 function intersects with pathways governing endocytosis, lysosomal metal recycling, and iron-responsive gene regulation.
NRAMP 2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SLC11A2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SLC11A2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SLC11A2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SLC11A2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.