NPM3 CRISPR/Cas9 KO Plasmid (m): sc-421944
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NPM3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NPM3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NPM3 CRISPR/Cas9 KO Plasmid (m) | sc-421944 | 20 µg | $397.00 |
Overview
Mouse Npm3 encodes nucleophosmin/nucleoplasmin 3 (NPM3), a nucleolar protein implicated in ribosome biogenesis and nucleolar homeostasis through regulation of rRNA processing and ribonucleoprotein assembly. NPM3 participates in chromatin-associated processes and nucleocytoplasmic dynamics by interacting with other nucleolar factors, linking nucleolar function to cell cycle progression and stress responses. Perturbation of nucleolar proteins in this pathway can influence proliferation control, genomic stability, and transcriptional programs relevant to oncogenic transformation and hematologic malignancy biology. As a nucleolar scaffold component, NPM3 provides a useful entry point for dissecting how nucleolar integrity modulates growth signaling and cellular adaptation to proteotoxic or genotoxic stress.
NPM3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Npm3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Npm3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Npm3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NPM3 protein expression.
This CRISPR knockout system enables efficient generation of Npm3-deficient cell models for investigation of NPM3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Npm3 exon(s) critical for NPM3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Npm3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NPM3 CRISPR/Cas9 KO Plasmid (m) and NPM3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Npm3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NPM3 HDR Plasmid (m) and NPM3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Npm3 homology arms to support homology-directed repair at defined Npm3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.