NPEPL1 Antibody (E-10): sc-515463
- NPEPL1 Antibody (E-10) is a mouse monoclonal IgG2a κ NPEPL1 antibody provided at 200 µg/ml
- raised against amino acids 55-112 mapping near the N-terminus of NPEPL1 of human origin
- NPEPL1 Antibody (E-10) is recommended for detection of NPEPL1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-NPEPL1 Antibody (E-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NPEPL1 Antibody (E-10) for WB applications. These reagents are now offered in bundles with NPEPL1 Antibody (E-10) (see ordering information below).
QUICK LINKS
SEE ALSO...
NPEPL1 Antibody (E-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects NPEPL1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NPEPL1 (E-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NPEPL1, also known as aminopeptidase-like 1, is a 523 amino acid protein that plays a crucial role in processing and degrading intracellular proteins by catalyzing the removal of unsubstituted N-terminal amino acids from various peptides. This function maintains cellular homeostasis and regulates protein turnover, essential for cellular function and stress response. NPEPL1 belongs to the peptidase M17 family and contains several zinc-binding sites important for enzymatic activity. NPEPL1 exists as three isoforms due to alternative splicing, allowing functional diversity in different cellular contexts. Widespread expression across tissues highlights NPEPL1′s importance in various physiological processes, including hormone regulation and intracellular protein metabolism.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NPEPL1 Antibody (E-10) References:
- Impact of the N-terminal amino acid on targeted protein degradation. | Meinnel, T., et al. 2006. Biol Chem. 387: 839-51. PMID: 16913833
- Biochemical characterization and structural prediction of a novel cytosolic leucyl aminopeptidase of the M17 family from Schizosaccharomyces pombe. | Herrera-Camacho, I., et al. 2007. FEBS J. 274: 6228-40. PMID: 18028193
- Peptide hydrolysis by the binuclear zinc enzyme aminopeptidase from Aeromonas proteolytica: a density functional theory study. | Chen, SL., et al. 2008. J Phys Chem B. 112: 2494-500. PMID: 18247603
- Aminopeptidases: towards a mechanism of action. | Taylor, A. 1993. Trends Biochem Sci. 18: 167-71. PMID: 8328016
- Aminopeptidases: structure and function. | Taylor, A. 1993. FASEB J. 7: 290-8. PMID: 8440407
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NPEPL1 Antibody (E-10) | sc-515463 | 200 µg/ml | $322.00 | |||
NPEPL1 Antibody (E-10): m-IgG Fc BP-HRP Bundle | sc-531379 | 200 µg Ab; 10 µg BP | $361.00 | |||
NPEPL1 Antibody (E-10): m-IgGκ BP-HRP Bundle | sc-525055 | 200 µg Ab, 40 µg BP | $361.00 | |||
NPEPL1 Antibody (E-10): m-IgG2a BP-HRP Bundle | sc-547777 | 200 µg Ab; 10 µg BP | $361.00 | |||
NPEPL1 Antibody (E-10) AC | sc-515463 AC | 500 µg/ml, 25% agarose | $424.00 | |||
NPEPL1 Antibody (E-10) HRP | sc-515463 HRP | 200 µg/ml | $322.00 | |||
NPEPL1 Antibody (E-10) FITC | sc-515463 FITC | 200 µg/ml | $336.00 | |||
NPEPL1 Antibody (E-10) PE | sc-515463 PE | 200 µg/ml | $349.00 | |||
NPEPL1 Antibody (E-10) Alexa Fluor® 488 | sc-515463 AF488 | 200 µg/ml | $364.00 | |||
NPEPL1 Antibody (E-10) Alexa Fluor® 546 | sc-515463 AF546 | 200 µg/ml | $364.00 | |||
NPEPL1 Antibody (E-10) Alexa Fluor® 594 | sc-515463 AF594 | 200 µg/ml | $364.00 | |||
NPEPL1 Antibody (E-10) Alexa Fluor® 647 | sc-515463 AF647 | 200 µg/ml | $364.00 | |||
NPEPL1 Antibody (E-10) Alexa Fluor® 680 | sc-515463 AF680 | 200 µg/ml | $364.00 | |||
NPEPL1 Antibody (E-10) Alexa Fluor® 790 | sc-515463 AF790 | 200 µg/ml | $364.00 |