NPAS2 Antibody (FT-68): sc-134404

NPAS2 Antibody (FT-68) is a mouse monoclonal IgG2a kappa light chain antibody that detects the NPAS2 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). NPAS2 (FT-68) antibody is available as a non-conjugated anti-NPAS2 antibody. Neuronal PAS domain protein 2 (NPAS2), also known as PAS 4 or MOP4, is a crucial transcription factor belonging to the basic helix-loop-helix-PER-ARNT-SIM (bHLH-PAS) family, which is characterized by a bHLH DNA binding domain and a PAS domain. NPAS2 is primarily expressed in neurons during the early stages of postnatal development, specifically mapping to chromosome 2p11.2-2q13. NPAS2 expression patterns align temporally with the development of learning and memory, and spatially associate with the frontal association and limbic forebrain pathways. NPAS2 plays a significant regulatory role in the development and maintenance of long-term memory and is essential for processing complex sensory information. Additionally, NPAS2 forms a transcriptionally active heterodimer with MOP3, which binds to specific DNA elements containing the CACGTGA sequence, thereby driving transcription from linked luciferase reporter genes. This interaction highlights NPAS2′s importance in gene regulation and potential implications in cognitive functions and neurodevelopmental processes.

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NPAS2 Antibody (FT-68) References:

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2. Characterization of the chicken serotonin N-acetyltransferase gene. Activation via clock gene heterodimer/E box interaction.  |  Chong, NW., et al. 2000. J Biol Chem. 275: 32991-8. PMID: 10931848
3. NPAS2 Regulation of Anxiety-Like Behavior and GABAA Receptors.  |  Ozburn, AR., et al. 2017. Front Mol Neurosci. 10: 360. PMID: 29163035
4. Circadian clock gene NPAS2 promotes reprogramming of glucose metabolism in hepatocellular carcinoma cells.  |  Yuan, P., et al. 2020. Cancer Lett. 469: 498-509. PMID: 31765736
5. Roles of NPAS2 in circadian rhythm and disease.  |  Peng, LU., et al. 2021. Acta Biochim Biophys Sin (Shanghai). 53: 1257-1265. PMID: 34415290
6. NPAS2 ameliorates myocardial ischaemia/reperfusion injury in rats via CX3CL1 pathways and regulating autophagy.  |  Huang, J., et al. 2021. Aging (Albany NY). 13: 20569-20584. PMID: 34460437
7. NPAS2 promotes aerobic glycolysis and tumor growth in prostate cancer through HIF-1A signaling.  |  Ma, S., et al. 2023. BMC Cancer. 23: 280. PMID: 36978001
8. Role of the Circadian Gas-Responsive Hemeprotein NPAS2 in Physiology and Pathology.  |  Murgo, E., et al. 2023. Biology (Basel). 12: PMID: 37887064
9. NPAS2 dampens chemo-sensitivity of lung adenocarcinoma cells by enhancing DNA damage repair.  |  Zhang, Y., et al. 2024. Cell Death Dis. 15: 101. PMID: 38291048
10. Molecular characterization of two mammalian bHLH-PAS domain proteins selectively expressed in the central nervous system.  |  Zhou, YD., et al. 1997. Proc Natl Acad Sci U S A. 94: 713-8. PMID: 9012850
11. Characterization of a subset of the basic-helix-loop-helix-PAS superfamily that interacts with components of the dioxin signaling pathway.  |  Hogenesch, JB., et al. 1997. J Biol Chem. 272: 8581-93. PMID: 9079689
12. The basic-helix-loop-helix-PAS orphan MOP3 forms transcriptionally active complexes with circadian and hypoxia factors.  |  Hogenesch, JB., et al. 1998. Proc Natl Acad Sci U S A. 95: 5474-9. PMID: 9576906