NOTCH2NL Antibody (39-Y) recommended for detection of NOTCH2NL of human origin by WB, IP, IF and ELISA

NOTCH2NL Antibody (39-Y): sc-100307

NOTCH2NL Antibody (39-Y) is rated 4.0 out of 5 by 2.
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Datasheets

    • Anti-NOTCH2NL Antibody (39-Y) is a mouse monoclonal IgG1 (kappa light chain) NOTCH2NL antibody provided at 100 µg/ml
    • raised against recombinant NOTCH2NL of human origin
    • recommended for detection of NOTCH2NL of human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

I am using NOTCH2NL (39-Y): sc-100307 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jenni
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Produced wonderful immunofluorescence cytoplasmic Produced wonderful immunofluorescence cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-04-05
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