Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
NOS1 Double Nickase Plasmid (h): sc-418493-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NOS1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NOS1 Double Nickase Plasmid (h) and NOS1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NOS1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: NOS1 Antibody (A-11): sc-5302
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NOS1 Double Nickase Plasmid (h) | sc-418493-NIC | 20 µg | $410.00 | |||
NOS1 Double Nickase Plasmid (h2) | sc-418493-NIC-2 | 20 µg | $410.00 |
Human NOS1 encodes neuronal nitric oxide synthase (nNOS), a calcium/calmodulin-regulated enzyme that generates nitric oxide from L-arginine and thereby modulates cGMP signaling. NOS1-derived nitric oxide regulates neurotransmission, synaptic plasticity, neurovascular coupling, and smooth muscle tone, with additional roles in redox homeostasis through reactive nitrogen species formation. In cells, NOS1 activity is integrated with NMDA receptor–dependent calcium influx, MAPK signaling, and scaffolded complexes that localize nNOS to specific subcellular microdomains. Dysregulated NOS1 signaling has been implicated in neurological and psychiatric phenotypes, pain processing, and cardiovascular regulation, making it a useful target for mechanistic studies of NO-dependent pathways.
NOS1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NOS1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NOS1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NOS1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NOS1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.