Thank you for your question. You can find our recommended ChIP protocol under this link:
https://www.scbt.com/resources/protocols/chromatin-immunoprecipitation-assays
The recommended amount of antibody for ChIP experiments is 1-2 μg (2,5-5 µl of normal mouse IgG: sc-2025) per 100-500 μg total protein (1 ml cell lysate).
Answered by: Tech Support Europe
Date published: 2020-06-15