normal mouse IgG normal unconjugated immunoglobulin for use as a negative control

normal mouse IgG: sc-2025

normal mouse IgG is rated 4.8 out of 5 by 65.
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Datasheets

    • provided at 200 µg in 0.5 ml volume
    • use as negative control for Western blotting, immunoprecipitation and immunohistochemistry applications
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Hi, I want to ask if this antibody can be used as isotype control for flow cytometry? Thanks!

Asked by: kf123
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2022-04-14

Can this antibody be modified into the no-sodium azide one? If yes, what would you do? Thank you!

Asked by: Anonymous
Thank you for your question. Please contact technical service for assistance with special order requests.
Answered by: Tech Support Europe
Date published: 2021-04-07

What is the molecular weight of sc-2025?

Asked by: prest
Thank you for your question. IgG antibodies have a molecular weight of 150.000 g/mol.
Answered by: Tech Support Europe
Date published: 2020-11-19

Is the antibody compatible with the secondary antibody (sc-516102)?

Asked by: ELis68
Thank you for your question. Normal mouse IgG: sc-2025 can be detected by m-IgGκ BP-HRP: sc-516102.
Answered by: Tech Support Europe
Date published: 2020-11-16

We have two different lots numbers for sc-2025.  Which is newer?  J1518 or G2020?  We are having an issue with G2020 giving us a positive signal in an immunoprecipitation that used to be negative and are trying to figure out what is going on.  

Asked by: Mars
Thank you for your question. Lot G2020 is the newer lot of these two. If you have any further questions or need any help with troubleshooting, please contact Technical Service.
Answered by: Technical Service 3
Date published: 2020-11-13

Hello, Can you please let me know how much dilution of normal mouse IgG: sc-2025 is recommended for ChIP experiments?

Asked by: Anonymous
Thank you for your question. You can find our recommended ChIP protocol under this link: https://www.scbt.com/resources/protocols/chromatin-immunoprecipitation-assays The recommended amount of antibody for ChIP experiments is 1-2 μg (2,5-5 µl of normal mouse IgG: sc-2025) per 100-500 μg total protein (1 ml cell lysate).
Answered by: Tech Support Europe
Date published: 2020-06-15

Chiedo con urgenza se le IgG normal si devono utilizzare per la tecnica Chip

Asked by: Maria Maddalena
Grazie per la sua domanda. Si prega di mettersi in contatto con il nostro servizio tecnico per organizzare una versione speciale di questo anticorpo senza proteine di supporto per ChIP.
Answered by: Tech Support Europe
Date published: 2019-06-14

What is the specific IgG isotype of this antibody?

Asked by: Cathy123
Thank you for your question. The normal mouse IgG is a polyclonal mixture of different isotypes, whereas the isotype specific controls (i.e normal mouse IgG1, IgG2a, etc) are monoclonals.
Answered by: Technical Support
Date published: 2019-05-10
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Rated 4 out of 5 by from Work well We use this IgG for ChIP and it works well in past years
Date published: 2022-12-06
Rated 5 out of 5 by from 研究生 师姐购买的的这个产品,但是我们都不清楚这个sc2025IgG是哪一个型啊,还是不论哪个型,它都可以作为对照IgG呢!求解答。我的鼠源单抗IgG是2a。
Date published: 2022-11-30
Rated 5 out of 5 by from It came in 3 days and it works perfectly I used this antibody as isotype control for Immunohistochemistry experiments and it works perfectly. The concentration needs to be adjusted to match the concentration of your primary antibody.
Date published: 2022-10-14
Rated 5 out of 5 by from 符合实验使用需求 用来标记荧光,没什么问题,非特异性吸附不高。用来标记荧光,没什么问题,非特异性吸附不高。用来标记荧光,没什么问题,非特异性吸附不高。
Date published: 2022-09-23
Rated 5 out of 5 by from 效果很好,性价比高 实验效果很好,价格实惠,发货及时。还有小样赠送,性价比非常高,以后尽量选择santa,做santa的忠实用户。
Date published: 2022-03-11
Rated 4 out of 5 by from 产品很不错 参考文献购买的,按大概的用量买了两管,使用了一管,目前跑出来的效果还不错。希望你们品牌的所有抗体也是一致的好用。
Date published: 2021-09-06
Rated 5 out of 5 by from Clean and convenient IgG control This IgG provides low unspecific binding, it is convenient for ChIP and for all kinds of IP experiments,great performance as control, recommended
Date published: 2021-05-19
Rated 5 out of 5 by from 功能出色 我半个月前购买了santa的抗体和protein A/G agarose,效果都不错,以后会继续购买的。
Date published: 2021-03-24
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