Nop5p/Nop56p Antibody (37C12): sc-57943

Nop5p/Nop56p Antibody (37C12) is a mouse monoclonal IgG1 antibody that detects Nop5p/Nop56p of Saccharomyces cerevisiae origin by immunoprecipitation (IP) and immunofluorescence (IF). Anti-Nop5p/Nop56p antibody (37C12) is available as the non-conjugated format. Nop5p/Nop56p (37C12) antibody recognizes a protein crucial in ribosomal biogenesis, specifically in early pre-rRNA processing, where Nop5p/Nop56p collaborates with Nop1p to facilitate 18S rRNA formation. This function ensures proper ribosome assembly, essential for protein synthesis in all living cells. Nop5p/Nop56p′s interaction with Nop1p maintains cellular function and viability, as defects in ribosomal biogenesis can lead to severe cellular dysfunction and disease. Nop5p/Nop56p also functions within box C/D small nucleolar ribonucleoprotein complexes, responsible for site-specific 2′-O-methylation of pre-rRNA, a critical modification for ribosomal RNA maturation. Anti-Nop5p/Nop56p antibody (37C12) effectively targets these proteins, making this reagent invaluable for researchers studying ribosome assembly and function in yeast.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Nop5p/Nop56p Antibody (37C12) References:

1. Synthesis and assembly of the box C+D small nucleolar RNPs.  |  Lafontaine, DL. and Tollervey, D. 2000. Mol Cell Biol. 20: 2650-9. PMID: 10733567
2. Multiple growth factor induction of a murine early response gene that complements a lethal defect in yeast ribosome biogenesis.  |  Nelson, SA., et al. 2000. J Biol Chem. 275: 13835-41. PMID: 10788506
3. Box C/D small nucleolar RNA trafficking involves small nucleolar RNP proteins, nucleolar factors and a novel nuclear domain.  |  Verheggen, C., et al. 2001. EMBO J. 20: 5480-90. PMID: 11574480
4. Structure and function of archaeal box C/D sRNP core proteins.  |  Aittaleb, M., et al. 2003. Nat Struct Biol. 10: 256-63. PMID: 12598892
5. Proteomic analysis of human Nop56p-associated pre-ribosomal ribonucleoprotein complexes. Possible link between Nop56p and the nucleolar protein treacle responsible for Treacher Collins syndrome.  |  Hayano, T., et al. 2003. J Biol Chem. 278: 34309-19. PMID: 12777385
6. In vitro RNP assembly and methylation guide activity of an unusual box C/D RNA, cis-acting archaeal pre-tRNA(Trp).  |  Bortolin, ML., et al. 2003. Nucleic Acids Res. 31: 6524-35. PMID: 14602911
7. Structural and thermodynamic evidence for a stabilizing role of Nop5p in S-adenosyl-L-methionine binding to fibrillarin.  |  Aittaleb, M., et al. 2004. J Biol Chem. 279: 41822-9. PMID: 15286083
8. The bipartite architecture of the sRNA in an archaeal box C/D complex is a primary determinant of specificity.  |  Hardin, JW. and Batey, RT. 2006. Nucleic Acids Res. 34: 5039-51. PMID: 16984968
9. Nucleolar KKE/D repeat proteins Nop56p and Nop58p interact with Nop1p and are required for ribosome biogenesis.  |  Gautier, T., et al. 1997. Mol Cell Biol. 17: 7088-98. PMID: 9372940