Nop1p Antibody (28F2): sc-57940
- Nop1p Antibody (28F2) is a mouse monoclonal IgG3 Nop1p antibody, cited in 6 publications, provided at 250 µl supernatant
- raised against a nuclear preparation of S. cerevisiae origin
- recommended for detection of Nop1p of S. cerevisiae origin by WB, IP and IF
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Nop1p Antibody (28F2). This work is in progress.
Nop1p Antibody (28F2) is a mouse monoclonal IgG3 antibody that detects Nop1p of Saccharomyces cerevisiae origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-Nop1p antibody (28F2) is available as a non-conjugated format, providing researchers with a reliable tool for studying this essential protein. Nop1p, also known as nucleolar protein 1, plays a critical role in pre-ribosomal RNA processing, which is vital for ribosome biogenesis. Nop1p is phylogenetically conserved and homologous to vertebrate fibrillarin, underscoring importance across species. Structurally, Nop1p consists of 327 amino acids and localizes within the nucleolus, specifically in regions resembling the dense fibrillar component of mammalian nucleoli. The presence of glycine/arginine-rich sequence repeats at the amino terminus of Nop1p is significant, as these motifs are often involved in protein-protein interactions and RNA binding, which are crucial for function in ribonucleoprotein particle assembly. Nop1p′s interaction with small nucleolar RNAs (snoRNAs) is essential for snoRNP formation, which are necessary for pre-rRNA modification and processing. Nop1p′s role in these processes highlights importance in maintaining cellular function and viability, making Nop1p monoclonal antibody (28F2) an invaluable resource for researchers investigating ribosome biogenesis and nucleolar dynamics.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Nop1p Antibody (28F2) References:
- cDNA cloning and sequencing of human fibrillarin, a conserved nucleolar protein recognized by autoimmune antisera. | Aris, JP. and Blobel, G. 1991. Proc Natl Acad Sci U S A. 88: 931-5. PMID: 1846968
- Evolutionary conservation of the human nucleolar protein fibrillarin and its functional expression in yeast. | Jansen, RP., et al. 1991. J Cell Biol. 113: 715-29. PMID: 2026646
- Isolation and sequencing of NOP1. A yeast gene encoding a nucleolar protein homologous to a human autoimmune antigen. | Henríquez, R., et al. 1990. J Biol Chem. 265: 2209-15. PMID: 2298745
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nop1p Antibody (28F2) | sc-57940 | 250 µl supernatant | $322.00 |