NOL11 Double Nickase Plasmid (h): sc-411835-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NOL11 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NOL11 Double Nickase Plasmid (h) and NOL11 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NOL11. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NOL11 Double Nickase Plasmid (h) | sc-411835-NIC | 20 µg | $410.00 | |||
NOL11 Double Nickase Plasmid (h2) | sc-411835-NIC-2 | 20 µg | $410.00 |
NOL11 encodes a nucleolar protein that supports ribosome biogenesis by participating in pre-rRNA transcription and early processing events required for small subunit maturation. It functions within nucleolar regulatory networks linked to RNA polymerase I activity, rRNA modification, and assembly of preribosomal particles, thereby influencing global translational capacity. Perturbation of NOL11 is expected to trigger nucleolar stress responses that intersect with cell-cycle control and genome surveillance pathways such as p53 signaling. Because dysregulated ribosome biogenesis is a common feature of proliferative disorders and ribosomopathies, NOL11 is frequently investigated in studies of growth control, proteostasis, and stress-adaptive transcriptional programs.
NOL11 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NOL11 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NOL11. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NOL11 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NOL11-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.