Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Nogo Double Nickase Plasmid (h): sc-400819-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nogo Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nogo Double Nickase Plasmid (h) and Nogo Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting RTN4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Nogo Antibody (C-4): sc-271878
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nogo Double Nickase Plasmid (h) | sc-400819-NIC | 20 µg | $410.00 | |||
Nogo Double Nickase Plasmid (h2) | sc-400819-NIC-2 | 20 µg | $410.00 |
RTN4 encodes Nogo, an endoplasmic reticulum and cell-surface–associated protein best known for regulating neurite outgrowth, axon guidance, and synaptic remodeling through interactions with Nogo receptor complexes and downstream RhoA/ROCK signaling. In the central nervous system, Nogo contributes to activity-dependent structural plasticity and limits regenerative sprouting after injury, linking RTN4 to pathways controlling cytoskeletal dynamics and growth cone motility. Beyond neurons, RTN4 participates in membrane curvature and ER tubule organization, influencing intracellular trafficking and stress responses. Dysregulated Nogo signaling has been studied in the context of neurodegeneration, demyelinating pathology, and other conditions where axonal connectivity and repair processes are perturbed.
Nogo Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the RTN4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within RTN4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt RTN4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of RTN4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.