NNT Antibody (43-R17): sc-100490

NNT Antibody (43-R17) is a mouse monoclonal IgG1 antibody that detects NNT in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NNT, or Nicotinamide nucleotide transhydrogenase, is a crucial 1086 amino acid multi-pass mitochondrial inner membrane protein that plays a vital role in cellular respiration by facilitating the transfer of a hydride ion from NADH to NADP+. This process is essential for maintaining the proton gradient across the inner mitochondrial membrane, which is harnessed by NNT to function as a proton pump. The ability of NNT to produce high concentrations of NADPH is particularly important, as NADPH is a key cofactor in various biosynthetic reactions and is critical for detoxifying free radicals, thereby protecting cells from oxidative stress. The homodimeric structure of NNT, with its distinct N-terminal and C-terminal domains belonging to the AlaDH/PNT family and the PNT beta subunit family respectively, underscores its complex functionality and importance in mitochondrial energy metabolism. Anti-NNT antibody (43-R17) is an invaluable tool for researchers studying mitochondrial function and the biochemical pathways involved in energy production and cellular defense mechanisms.

The preferred NNT antibody is NNT Antibody (B-3): sc-390236.