NMD3 Antibody (A-5): sc-515426
- NMD3 Antibody (A-5) is a mouse monoclonal IgG1 κ NMD3 antibody provided at 200 µg/ml
- raised against amino acids 320-377 mapping within an internal region of NMD3 of human origin
- NMD3 Antibody (A-5) is recommended for detection of NMD3 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-NMD3 Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for NMD3 Antibody (A-5) for WB applications. These reagents are now offered in bundles with NMD3 Antibody (A-5) (see ordering information below).
QUICK LINKS
SEE ALSO...
NMD3 Antibody (A-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects NMD3 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). NMD3 (A-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. NMD3 plays a crucial role in the nuclear export of the 60S ribosomal subunit, which is essential for proper ribosome assembly and subsequent protein synthesis. This process is vital for cellular function, as ribosomes translate messenger RNA into proteins necessary for various cellular activities. NMD3 contains a CRM-1-dependent leucine-rich nuclear export signal and a complex nuclear localization signal, highlighting NMD3′s importance in regulating ribosomal subunit transport. The gene encoding NMD3 is located on human chromosome 3, a region associated with over 1,100 genes, including several implicated in human cancers, underscoring NMD3′s potential significance in both normal cellular processes and disease states.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
NMD3 Antibody (A-5) References:
- 60S pre-ribosome formation viewed from assembly in the nucleolus until export to the cytoplasm. | Nissan, TA., et al. 2002. EMBO J. 21: 5539-47. PMID: 12374754
- Nuclear export of ribosomal subunits. | Johnson, AW., et al. 2002. Trends Biochem Sci. 27: 580-5. PMID: 12417134
- [New tumor suppressor genes in hot spots of human chromosome 3: new methods of identification]. | Braga, EA., et al. 2003. Mol Biol (Mosk). 37: 194-211. PMID: 12723467
- Biogenesis and nuclear export of ribosomal subunits in higher eukaryotes depend on the CRM1 export pathway. | Thomas, F. and Kutay, U. 2003. J Cell Sci. 116: 2409-19. PMID: 12724356
- Coordinated nuclear export of 60S ribosomal subunits and NMD3 in vertebrates. | Trotta, CR., et al. 2003. EMBO J. 22: 2841-51. PMID: 12773398
- The DNA sequence, annotation and analysis of human chromosome 3. | Muzny, DM., et al. 2006. Nature. 440: 1194-8. PMID: 16641997
- Establishment and characterization of two uveal melanoma cell lines derived from tumors with loss of one chromosome 3. | Nareyeck, G., et al. 2006. Exp Eye Res. 83: 858-64. PMID: 16750193
- Novel interaction of the 60S ribosomal subunit export adapter Nmd3 at the nuclear pore complex. | West, M., et al. 2007. J Biol Chem. 282: 14028-37. PMID: 17347149
- Joining the interface: a site for Nmd3 association on 60S ribosome subunits. | Oeffinger, M. 2010. J Cell Biol. 189: 1071-3. PMID: 20584913
- CRM1 and its ribosome export adaptor NMD3 localize to the nucleolus and affect rRNA synthesis. | Bai, B., et al. 2013. Nucleus. 4: 315-25. PMID: 23782956
- Nmd3 is a structural mimic of eIF5A, and activates the cpGTPase Lsg1 during 60S ribosome biogenesis. | Malyutin, AG., et al. 2017. EMBO J. 36: 854-868. PMID: 28179369
- GRC5 and NMD3 function in translational control of gene expression and interact genetically. | Karl, T., et al. 1999. Curr Genet. 34: 419-29. PMID: 9933353
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NMD3 Antibody (A-5) | sc-515426 | 200 µg/ml | $322.00 | |||
NMD3 Antibody (A-5): m-IgG Fc BP-HRP Bundle | sc-531360 | 200 µg Ab; 10 µg BP | $361.00 | |||
NMD3 Antibody (A-5): m-IgGκ BP-HRP Bundle | sc-525032 | 200 µg Ab, 40 µg BP | $361.00 | |||
NMD3 Antibody (A-5): m-IgG1 BP-HRP Bundle | sc-544504 | 200 µg Ab; 20 µg BP | $361.00 | |||
NMD3 Antibody (A-5) AC | sc-515426 AC | 500 µg/ml, 25% agarose | $424.00 | |||
NMD3 Antibody (A-5) HRP | sc-515426 HRP | 200 µg/ml | $322.00 | |||
NMD3 Antibody (A-5) FITC | sc-515426 FITC | 200 µg/ml | $336.00 | |||
NMD3 Antibody (A-5) PE | sc-515426 PE | 200 µg/ml | $349.00 | |||
NMD3 Antibody (A-5) Alexa Fluor® 488 | sc-515426 AF488 | 200 µg/ml | $364.00 | |||
NMD3 Antibody (A-5) Alexa Fluor® 546 | sc-515426 AF546 | 200 µg/ml | $364.00 | |||
NMD3 Antibody (A-5) Alexa Fluor® 594 | sc-515426 AF594 | 200 µg/ml | $364.00 | |||
NMD3 Antibody (A-5) Alexa Fluor® 647 | sc-515426 AF647 | 200 µg/ml | $364.00 | |||
NMD3 Antibody (A-5) Alexa Fluor® 680 | sc-515426 AF680 | 200 µg/ml | $364.00 | |||
NMD3 Antibody (A-5) Alexa Fluor® 790 | sc-515426 AF790 | 200 µg/ml | $364.00 |