Nlk Antibody (B-5): sc-48361

Nlk Antibody (B-5) is a mouse monoclonal IgG2a antibody that detects Nlk protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Nlk antibody (B-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nlk protein, also known as Nemo-Like Kinase, plays a crucial role in cellular signaling pathways, particularly in the regulation of gene expression and cell proliferation. Nlk functions as a nuclear protein that can undergo autophosphorylation, which is essential for activity and function. Nlk is involved in the modulation of various signaling cascades, including those activated by growth factors and hormones, and dysregulation has been implicated in several diseases, including cancer. Nlk′s ability to interact with other signaling molecules enhances importance in maintaining cellular homeostasis and responding to external stimuli. Understanding the function and interactions of Nlk is vital for elucidating role in health and disease, making Nlk (B-5) monoclonal antibody an invaluable tool for researchers studying these pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Nlk Antibody (B-5) References:

1. ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ.  |  Haycock, JW., et al. 1992. Proc Natl Acad Sci U S A. 89: 2365-9. PMID: 1347949
2. Purification of a murine protein-tyrosine/threonine kinase that phosphorylates and activates the Erk-1 gene product: relationship to the fission yeast byr1 gene product.  |  Crews, CM. and Erikson, RL. 1992. Proc Natl Acad Sci U S A. 89: 8205-9. PMID: 1381507
3. The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product.  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
4. Identification of multiple extracellular signal-regulated kinases (ERKs) with antipeptide antibodies.  |  Boulton, TG. and Cobb, MH. 1991. Cell Regul. 2: 357-71. PMID: 1654126
5. Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase.  |  Boulton, TG., et al. 1991. Biochemistry. 30: 278-86. PMID: 1846291
6. ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF.  |  Boulton, TG., et al. 1991. Cell. 65: 663-75. PMID: 2032290
7. Nemo-like kinase, a multifaceted cell signaling regulator.  |  Ishitani, T. and Ishitani, S. 2013. Cell Signal. 25: 190-7. PMID: 23000342
8. Nemo-like kinase regulates postnatal skeletal homeostasis.  |  Canalis, E., et al. 2014. J Cell Physiol. 229: 1736-43. PMID: 24664870
9. The emerging role of Nemo-like kinase (NLK) in the regulation of cancers.  |  Huang, Y., et al. 2015. Tumour Biol. 36: 9147-52. PMID: 26427665
10. Reduction of nemo-like kinase increases lysosome biogenesis and ameliorates TDP-43-related neurodegeneration.  |  Tejwani, L., et al. 2023. J Clin Invest. 133: PMID: 37384409
11. Nemo-like kinase blocks myeloid differentiation by targeting tumor suppressor C/EBPα in AML.  |  Singh, AK., et al. 2024. FEBS J. 291: 4539-4557. PMID: 39110129

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nlk Antibody (B-5) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-Nlk Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nlk Antibody (B-5) Specifications

Alternate Product Name: Nemo Like Kinase

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids: 416-515

Epitope Target: Nlk

Epitope Species: human

Product Reactivity/Detection: Nlk

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for Nlk Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin