Nkx-6.1 Antibody (5B8): sc-130385
- Nkx-6.1 Antibody (5B8) is a mouse monoclonal IgG2a κ Nkx-6.1 antibody, cited in 1 publications, provided at 100 µg/ml
- raised against a full length recombinant protein Nkx-6.1 of human origin
- recommended for detection of Nkx-6.1 of mouse, rat and human origin by WB and IP
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Nkx-6.1 Antibody (5B8). This work is in progress.
Nkx-6.1 Antibody (5B8) is a mouse monoclonal IgG2a kappa light chain antibody that detects Nkx-6.1 protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). Anti-Nkx-6.1 antibody (5B8) is available as the non-conjugated format. Nkx-6.1 plays a crucial role in the development and function of the pancreas, particularly in regulating insulin-producing beta cells within the islets of Langerhans. Nkx-6.1 is essential for maintaining the identity and function of these cells, promoting their differentiation and regulating the expression of other key genes involved in insulin production, such as Pdx-1 and Glut2. Nkx-6.1′s interaction with these genes is vital for proper glucose homeostasis, and dysregulation has been implicated in diabetes and other metabolic disorders. Through binding to specific DNA sequences, Nkx-6.1 orchestrates the transcriptional repression of genes not required for beta cell function, ensuring cells can effectively respond to physiological demands. Nkx-6.1′s ability to integrate signals from developmental cues, such as sonic hedgehog, further underscores its importance in both embryonic development and adult pancreatic function.
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Nkx-6.1 Antibody (5B8) References:
- Homeobox gene Nkx2.2 and specification of neuronal identity by graded Sonic hedgehog signalling. | Briscoe, J., et al. 1999. Nature. 398: 622-7. PMID: 10217145
- Cloning and DNA-binding properties of the rat pancreatic beta-cell-specific factor Nkx6.1. | Jørgensen, MC., et al. 1999. FEBS Lett. 461: 287-94. PMID: 10567713
- NKX2 gene expression in neuroectoderm but not in mesendodermally derived structures depends on sonic hedgehog in mouse embryos. | Pabst, O., et al. 2000. Dev Genes Evol. 210: 47-50. PMID: 10603087
- The homeodomain of Nkx2.2 carries two cooperatively acting nuclear localization signals. | Hessabi, B., et al. 2000. Biochem Biophys Res Commun. 270: 695-700. PMID: 10772886
- Beta-cell differentiation factor Nkx6.1 contains distinct DNA binding interference and transcriptional repression domains. | Mirmira, RG., et al. 2000. J Biol Chem. 275: 14743-51. PMID: 10799563
- Evidence for the differential regulation of Nkx-6.1 expression in the ventral spinal cord and foregut by Shh-dependent and -independent mechanisms. | Cai, J., et al. 2000. Genesis. 27: 6-11. PMID: 10862150
- Transcriptional and translational regulation of beta-cell differentiation factor Nkx6.1. | Watada, H., et al. 2000. J Biol Chem. 275: 34224-30. PMID: 10938085
- Pdx1 level defines pancreatic gene expression pattern and cell lineage differentiation. | Wang, H., et al. 2001. J Biol Chem. 276: 25279-86. PMID: 11309388
- Homeobox gene product Nkx 6.1 immunoreactivity in nuclei of endocrine cells of rat and mouse stomach. | Oster, A., et al. 1998. J Histochem Cytochem. 46: 717-21. PMID: 9603782
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nkx-6.1 Antibody (5B8) | sc-130385 | 100 µg/ml | $339.00 |