Nkx-3.1 Antibody (A-3): sc-393190

Nkx-3.1 Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects Nkx-3.1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Nkx-3.1 antibody (A-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Nkx-3.1, a member of the homeobox gene family, plays a crucial role in prostate development and is primarily expressed in the prostate gland, where Nkx-3.1 regulates cellular responses to sonic hedgehog (Shh) signaling. This regulation is vital for maintaining the balance between cell proliferation and differentiation within the prostatic epithelium, which is essential for normal prostate function and development. Nkx-3.1 is expressed at lower levels in other tissues, such as the heart and gut, contributing to the regulation of glandular epithelium proliferation and duct formation in both the prostate and minor salivary glands. Nkx-3.1 preferentially binds to the TAAGTA sequence, a unique characteristic not observed in other NK class homeoproteins. The human Nkx-3.1 gene is located on chromosome 8q21, a region often associated with loss of heterozygosity in various cancers, making Nkx-3.1 a potential marker for distinguishing between benign and malignant prostate epithelium.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Nkx-3.1 Antibody (A-3) References:

1. DNA-binding sequence of the human prostate-specific homeodomain protein NKX3.1.  |  Steadman, DJ., et al. 2000. Nucleic Acids Res. 28: 2389-95. PMID: 10871372
2. Targeted disruption of the Nkx3.1 gene in mice results in morphogenetic defects of minor salivary glands: parallels to glandular duct morphogenesis in prostate.  |  Schneider, A., et al. 2000. Mech Dev. 95: 163-74. PMID: 10906459
3. Nkx3.1, a murine homolog of Ddrosophila bagpipe, regulates epithelial ductal branching and proliferation of the prostate and palatine glands.  |  Tanaka, M., et al. 2000. Dev Dyn. 219: 248-60. PMID: 11002344
4. Loss of NKX3.1 expression in human prostate cancers correlates with tumor progression.  |  Bowen, C., et al. 2000. Cancer Res. 60: 6111-5. PMID: 11085535
5. Expression studies and mutational analysis of the androgen regulated homeobox gene NKX3.1 in benign and malignant prostate epithelium.  |  Ornstein, DK., et al. 2001. J Urol. 165: 1329-34. PMID: 11257711
6. Gene expression profiles in the PC-3 human prostate cancer cells induced by NKX3.1.  |  Zhang, P., et al. 2010. Mol Biol Rep. 37: 1505-12. PMID: 19462257
7. Androgen regulation of the prostatic tumour suppressor NKX3.1 is mediated by its 3' untranslated region.  |  Thomas, MA., et al. 2010. Biochem J. 425: 575-83. PMID: 19886863
8. Variant NKX3.1 and Serum IGF-1: Investigation of Interaction in Prostate Cancer.  |  Muhlbradt, E., et al. 2013. Genes Cancer. 4: 535-45. PMID: 24386513
9. Transcriptional regulation of the Nkx3.1 gene in prostate luminal stem cell specification and cancer initiation via its 3' genomic region.  |  Xie, Q. and Wang, ZA. 2017. J Biol Chem. 292: 13521-13530. PMID: 28679531
10. Determination of ERG(+), EZH2, NKX3.1, and SPINK-1 subtypes to evaluate their association with clonal origin and disease progression in multifocal prostate cancer.  |  Segura-Moreno, YY., et al. 2023. Cancer Rep (Hoboken). 6: e1728. PMID: 36199157
11. tinman and bagpipe: two homeo box genes that determine cell fates in the dorsal mesoderm of Drosophila.  |  Azpiazu, N. and Frasch, M. 1993. Genes Dev. 7: 1325-40. PMID: 8101173
12. Tissue-specific expression of murine Nkx3.1 in the male urogenital system.  |  Sciavolino, PJ., et al. 1997. Dev Dyn. 209: 127-38. PMID: 9142502

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Nkx-3.1 Antibody (A-3) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-Nkx-3.1 Antibody (A-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Nkx-3.1 Antibody (A-3) Specifications

Alternate Product Name: Nkx-3.1

Product Clone Name: A-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-50

Epitope Target: Nkx-3.1

Epitope Species: human

Product Reactivity/Detection: Nkx-3.1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for Nkx-3.1 Antibody (A-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent